ArticleInternational journal of nanomedicine
Cytotoxic effects of ZnO nanoparticles on mouse testicular cells.
Article in International journal of nanomedicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 121 citations in OpenAlex.
- Impact of Nanoparticles on Male Fertility: What Do We Really Know? A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Efficacy of zinc oxide nanoparticles in chemical castration of male Wistar rats.Scientific reports · 2025Article
- Protective Effect of Soybean Isoflavone (SI) on Testicular Damages Induced by Zinc Oxide Nanoparticles (ZnONPs) in Male Mice.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Evaluation of chemical castration by intra-testicular injection of zinc-doped carbon dots in mature rats.Veterinary research forum : an international quarterly journal · 2025Article
- ZnO nanoparticles induce acute arrhythmia and heart failure in mice by disturbing cardiac ion channels.Frontiers in cardiovascular medicine · 2025Article
- A comprehensive review on potential role of selenium, selenoproteins and selenium nanoparticles in male fertility.Heliyon · 2024Review
- ZnO NPs induce miR-342-5p mediated ferroptosis of spermatocytes through the NF-κB pathway in mice.Journal of nanobiotechnology · 2024Article
- Reproductive toxicity perspectives of nanoparticles: an update.Toxicology research · 2024Review
- Toxicological inhalation studies in rats to substantiate grouping of zinc oxide nanoforms.Particle and fibre toxicology · 2024Article
- Determinants and mechanisms of inorganic nanoparticle translocation across mammalian biological barriers.Archives of toxicology · 2023Review
- TiBiology direct · 2023Article
- Vitamins A, C, and E Exert Anti-apoptotic Function in the Testis of Rats After Exposure to Zinc Oxide Nanoparticles.Chonnam medical journal · 2023Article
- Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish.Antioxidants (Basel, Switzerland) · 2022Article
- Exposure to zinc oxide nanoparticles affects testicular structure, reproductive development and spermatogenesis in parental and offspring male rats.Annals of translational medicine · 2022Article
- Effects of iron oxide nanoparticles as TJournal of nanobiotechnology · 2022Article
- Switching to nanonutrients for sustaining agroecosystems and environment: the challenges and benefits in moving up from ionic to particle feeding.Journal of nanobiotechnology · 2022Review
- Comprehensive review on the positive and negative effects of various important regulators on male spermatogenesis and fertility.Frontiers in nutrition · 2022Review
- Attributes of oxidative stress in the reproductive toxicity of nickel oxide nanoparticles in male rats.Environmental science and pollution research international · 2022Article
- Impairment of sperm efficiency in mice following short-term nano-titanium dioxide exposure: An experimental study.International journal of reproductive biomedicine · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model.
methodsZnO NPs with a crystalline size of 70 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and atomic force microscopy. The cytotoxicity of the ZnO NPs was examined in vitro on Leydig cell and Sertoli cell lines, and in vivo on the testes of CD1 mice injected with single doses of ZnO NPs.
resultsZnO NPs were internalized by Leydig cells and Sertoli cells, and this resulted in cytotoxicity in a time- and dose-dependent manner through the induction of apoptosis. Apoptosis likely occurred as a consequence of DNA damage (detected as γ-H2AX and RAD51 foci) caused by increase in reactive oxygen species associated with loss of mitochondrial membrane potential. In addition, injection of ZnO NPs in male mice caused structural alterations in the seminiferous epithelium and sperm abnormalities.
conclusionThese results demonstrate that ZnO NPs have the potential to induce apoptosis in testicular cells likely through DNA damage caused by reactive oxygen species, with possible adverse consequences for spermatogenesis and therefore, male fertility. This suggests that evaluating the potential impacts of engineered NPs is essential prior to their mass production, to address both the environmental and human health concerns and also to develop sustainable and safer nanomaterials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.