Evidence map›Paper›PMID 27784934›Full record

ReviewIndian journal of microbiology2016

High Throughput Sequencing: An Overview of Sequencing Chemistry.

Sheetal Ambardar, Rikita Gupta, Deepika Trakroo, Rup Lal, Jyoti Vakhlu

Abstract readReview
In one paragraph

Review in Indian journal of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
75citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

75 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

15 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sheetal AmbardarMetagenomics Laboratory, School of Biotechnology, University of Jammu, Jammu, J&K India ; Centre for Cellular and Molecular Platform, National Centre for Biological Sciences, TIFR Bangalore, Bangalore, India ; Institute of Trans-Disciplinary Health Sciences and Technology, Trans-Disciplinary University, Bangalore, 560064 India.
Rikita GuptaMetagenomics Laboratory, School of Biotechnology, University of Jammu, Jammu, J&K India.
Deepika TrakrooMetagenomics Laboratory, School of Biotechnology, University of Jammu, Jammu, J&K India.
Rup LalMolecular Biology Laboratory, Department of Zoology, South Campus, University of Delhi, Delhi, India.
Jyoti VakhluMetagenomics Laboratory, School of Biotechnology, University of Jammu, Jammu, J&K India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present century sequencing is to the DNA science, what gel electrophoresis was to it in the last century. From 1977 to 2016 three generation of the sequencing technologies of various types have been developed. Second and third generation sequencing technologies referred commonly to as next generation sequencing technology, has evolved significantly with increase in sequencing speed, decrease in sequencing cost, since its inception in 2004. GS FLX by 454 Life Sciences/Roche diagnostics, Genome Analyzer, HiSeq, MiSeq and NextSeq by Illumina, Inc., SOLiD by ABI, Ion Torrent by Life Technologies are various type of the sequencing platforms available for second generation sequencing. The platforms available for the third generation sequencing are Helicos™ Genetic Analysis System by SeqLL, LLC, SMRT Sequencing by Pacific Biosciences, Nanopore sequencing by Oxford Nanopore's, Complete Genomics by Beijing Genomics Institute and GnuBIO by BioRad, to name few. The present article is an overview of the principle and the sequencing chemistry of these high throughput sequencing technologies along with brief comparison of various types of sequencing platforms available.

Indexed as

High throughput sequencingNGSSecond generation sequencingSequencing platformsThird generation sequencing

Identifiers

PMID27784934
PMCPMC5061697

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.