Trial reportJournal of translational medicine2016
Intraoperative hemidiaphragm electrical stimulation reduces oxidative stress and upregulates autophagy in surgery patients undergoing mechanical ventilation: exploratory study.
Trial report in Journal of translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 3 syntheses or guidelines pooled it, 43 citations in OpenAlex.
- Pooled it
- Outcomes in critically ill patients after diaphragmatic stimulation on ventilator-induced diaphragmatic dysfunction: a systematic review.European journal of physical and rehabilitation medicine · 2023Pooled it
- Research Progress of Electrical Stimulation in Ischemic Heart Disease.Frontiers in cardiovascular medicine · 2021Pooled it
- Effect of external diaphragmatic pacing therapy on patients with chronic cor pulmonale: a randomized, controlled trial.The Journal of international medical research · 2020Trial
- Noninvasive Electromagnetic Phrenic Nerve Stimulation in Critically Ill Patients: A Feasibility Study.Chest · 2024Article
- Phrenic nerve stimulation to prevent diaphragmatic dysfunction and ventilator-induced lung injury.Intensive care medicine experimental · 2023Review
- Temporary diaphragm pacing for patients at risk of prolonged mechanical ventilation after extensive aortic repair.Journal of vascular surgery cases and innovative techniques · 2023Article
- Intraoperative Hemi-Diaphragm Electrical Stimulation Demonstrates Attenuated Mitochondrial Function without Change in Oxidative Stress in Cardiothoracic Surgery Patients.Antioxidants (Basel, Switzerland) · 2023Article
- Phrenic nerve stimulation in an ovine model with temporary removable pacing leads.Journal of thoracic disease · 2022Article
- Altered expression of microRNAs in the rat diaphragm in a model of ventilator-induced diaphragm dysfunction after controlled mechanical ventilation.BMC genomics · 2021Article
- Diaphragmatic dysfunction associated with invasive mechanical ventilation in critically ill adult patientsRevista de la Facultad de Ciencias Medicas (Cordoba, Argentina) · 2021Article
- Article
- Temporary Transvenous Diaphragmatic Neurostimulation in Prolonged Mechanically Ventilated Patients: A Feasibility Trial (RESCUE 1).Critical care explorations · 2020Article
- Induction of autophagy protects human dental pulp cells from lipopolysaccharide-induced pyroptotic cell death.Experimental and therapeutic medicine · 2020Article
- Article
- Article
- Inspiratory Muscle Training in Patients with Prolonged Mechanical Ventilation: Narrative Review.Cardiopulmonary physical therapy journal · 2019Article
- Diaphragm Atrophy and Weakness in the Absence of Mitochondrial Dysfunction in the Critically Ill.American journal of respiratory and critical care medicine · 2017Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMechanical ventilation (MV) during a cardio-thoracic surgery contributes to diaphragm muscle dysfunction that impairs weaning and can lead to the ventilator- induced diaphragm dysfunction. Especially, it is critical in older adults who have lower muscle reparative capacity following MV. Reports have shown that the intraoperative intermittent hemidiaphragm electrical stimulation can maintain and/or improve post-surgery diaphragm function. In particular, from a molecular point of view, intermittent ES may reduce oxidative stress and increase regulatory autophagy levels, and therefore improve diaphragm function in animal studies. We have recently shown in humans that intraoperative ES attenuates mitochondrial dysfunction and force decline in single diaphragm muscle fibers. The aim of this study was to investigate an effect of ES on oxidative stress, antioxidant status and autophagy biomarker levels in the human diaphragm during surgery.
methodsOne phrenic nerve was simulated with an external cardiac pacer in operated older subjects (62.4 ± 12.9 years) (n = 8) during the surgery. The patients received 30 pulses per min every 30 min. The muscle biopsy was collected from both hemidiaphragms and frozen for further analyses. 4-hydroxynonenal (4-HNE), an oxidative stress marker, and autophagy marker levels (Beclin-1 and the ratio of microtubule-associated protein light chain 3, I and II-LC3 II/I) protein concentrations were detected by the western blot technique. Antioxidant enzymatic activity copper-zinc (CuZnSOD) and manganese (MnSOD) superoxide dismutase were analyzed.
resultsLevels of lipid peroxidation (4-HNE) were significantly lower in the stimulated side (p < 0.05). The antioxidant enzyme activities (CuZnSOD and MnSOD) in the stimulated side of the diaphragm were not different than in the unstimulated side (p > 0.05). Additionally, the protein concentrations of Beclin-1 and the LC3 II/I ratio were higher in the stimulated side (p < 0.05).
conclusionThese results suggest that the intraoperative electrical stimulation decreases oxidative stress levels and upregulates autophagy levels in the stimulated hemidiaphragm. These results may contribute future studies and clinical applications on reducing post-operative diaphragm dysfunction.
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