Evidence map›Paper›PMID 27784267›Full record

ArticleBMC developmental biology2016

Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis.

Andrew Robson, Nick D L Owens, Susan J Baserga, Mustafa K Khokha, John N Griffin

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Xenbase: Facilitating the Use ofFrontiers in physiology · 2019
    Review
  8. Review
  9. Development (Cambridge, England) · 2018
    Article
  10. Article
  11. The complete structure of the small-subunit processome.Nature structural & molecular biology · 2017
    Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Andrew RobsonProgram in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA.
Nick D L OwensThe Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, London, NW7 1AA, UK.
Susan J BasergaDepartments of Genetics, Molecular Biophysics and Biochemistry, and Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, 06520, USA.
Mustafa K KhokhaProgram in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA.
John N GriffinProgram in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA. J.Griffin@yale.edu.
Pediatrics and Genetics · USYale University · USThe Francis Crick Institute · GB

Funding

A system approach to the analysis of Heterotaxy Candiate GenesR01HD081379 · NICHD · YALE UNIVERSITY · PI KHOKHA, MUSTAFA K · 2014 to 2018
$2.7M
Cardiac phenotyping of CHD candidate genes in XenopusR33HL120783 · NHLBI · YALE UNIVERSITY · PI BRUECKNER, MARTINA, KHOKHA, MUSTAFA K · 2016 to 2018
$1.6M
Key factors in human ribosome biogenesisR01GM115710 · NIGMS · YALE UNIVERSITY · PI BASERGA, SUSAN J · 2015 to 2018
$1.6M
NHLBI NIH HHS R33 HL120783NICHD NIH HHS R01 HD081379NIGMS NIH HHS R01 GM115710
6 · The paper itself

Abstract

backgroundBecause ribosomes are ubiquitously required for protein production, it was long assumed that any inherited defect in ribosome manufacture would be embryonically lethal. However, several human congenital diseases have been found to be associated with mutations in ribosome biogenesis factors. Surprisingly, despite the global requirement for ribosomes, these "ribosomopathies" are characterized by distinct and tissue specific phenotypes. The reasons for such tissue proclivity in ribosomopathies remain mysterious but may include differential expression of ribosome biogenesis factors in distinct tissues.

methodsHere we use in situ hybridization of labeled antisense mRNA probes and ultra high temporal resolution RNA-Seq data to examine and compare expression of 13 disease associated ribosome biogenesis factors at six key stages in Xenopus tropicalis development.

resultsRather than being ubiquitously expressed during development, mRNAs of all examined ribosome biogenesis factors were highly enriched in specific tissues, including the cranial neural crest and ventral blood islands. Interestingly, expression of ribosome biogenesis factors demonstrates clear differences in timing, transcript number and tissue localization.

conclusionRibosome biogenesis factor expression is more spatiotemporally regulated during embryonic development than previously expected and correlates closely with many of the common ribosomopathy phenotypes. Our findings provide information on the dynamic use of ribosome production machinery components during development and advance our understanding of their roles in disease.

Indexed as

AnimalsEmbryonic DevelopmentGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Regulatory NetworksIn Situ HybridizationOrgan SpecificityRibosomesSequence Analysis, RNAXenopusXenopus ProteinsXenopus ProteinsDevelopmentDiamond-Blackfan anemiaNorth American Childhood CirrhosisRibosomeRibosome biogenesisRibosomopathyRPLRPSTCOF1UTPXenopus

Identifiers

PMID27784267
PMCPMC5081970
OpenAlexW2541860496

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.