ArticleCurrent genetics2017
Isolation of conditional mutations in genes essential for viability of Cryptococcus neoformans.
Article in Current genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Commensal yeast Malassezia produces tryptophan metabolites to promote tissue homeostasis via the aryl hydrocarbon receptor in mice.Nature microbiology · 2026Article
- Malassezia responds to environmental pH signals through the conserved Rim/Pal pathway.bioRxiv : the preprint server for biology · 2024Article
- Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast,Molecular biology of the cell · 2024Article
- Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optimizing Transformation Frequency ofJournal of fungi (Basel, Switzerland) · 2021Review
- Molecular Tools for the Yeast Papiliotrema terrestris LS28 and Identification of Yap1 as a Transcription Factor Involved in Biocontrol Activity.Applied and environmental microbiology · 2021Article
- Solid-state NMR spectroscopy identifies three classes of lipids inThe Journal of biological chemistry · 2020Article
- Genetic transformation ofeLife · 2020Article
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- Expression of aFrontiers in cellular and infection microbiology · 2020Article
- Approaches for Genetic Discoveries in the Skin Commensal and PathogenicFrontiers in cellular and infection microbiology · 2020Review
- Advancing Functional Genetics ThroughGenetics · 2019Article
- Proteomic and Metabolomic Characteristics of Extremophilic Fungi Under Simulated Mars Conditions.Frontiers in microbiology · 2019Article
- Creation, characterization and utilization of Cryptococcus neoformans mutants sensitive to micafungin.Current genetics · 2017Article
- FKBP12-Dependent Inhibition of Calcineurin Mediates Immunosuppressive Antifungal Drug Action inmBio · 2017Article
- Nutritional Requirements and Their Importance for Virulence of Pathogenic Cryptococcus Species.Microorganisms · 2017Review
- A silver bullet in a golden age of functional genomics: the impact ofFungal biology and biotechnology · 2017Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
Discovering the genes underlying fundamental processes that enable cells to live and reproduce is a technical challenge, because loss of gene function in mutants results in organisms that cannot survive. This study describes a forward genetics method to identify essential genes in fungi, based on the propensity for Agrobacterium tumefaciens to insert T-DNA molecules into the promoters or 5' untranslated regions of genes and by placing a conditional promoter within the T-DNA. Insertions of the promoter of the GAL7 gene were made in the human pathogen Cryptococcus neoformans. Nine strains of 960 T-DNA insertional mutants screened grew on media containing galactose, but had impaired growth on media containing glucose, which suppresses expression from GAL7. T-DNA insertions were found in the homologs of IDI1, MRPL37, NOC3, NOP56, PRE3 and RPL17, all of which are essential in ascomycete yeasts Saccharomyces cerevisiae or Schizosaccharomyces pombe. Altering the carbon source in the medium provided a system to identify phenotypes in response to stress agents. The pre3 proteasome subunit mutant was further characterized. The T-DNA insertion and phenotype co-segregate in progeny from a cross, and the growth defect is complemented by the reintroduction of the wild type gene into the insertional mutant. A deletion allele was generated in a diploid strain, this heterozygous strain was sporulated, and analysis of the progeny provided additional genetic evidence that PRE3 is essential. The experimental design is applicable to other fungi and has other forward genetic applications such as to isolate over-expression suppressors or enhance the production of traits of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.