ArticleBMC genomics2016
Comparison between two amplicon-based sequencing panels of different scales in the detection of somatic mutations associated with gastric cancer.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 34 citations in OpenAlex.
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- Differences in Somatic Mutation Profiles between Korean Gastric Cancer and Gastric Adenoma Patients.Journal of clinical medicine · 2021Article
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- Targeted Next-Generation Sequencing of Liquid Biopsy Samples from Patients with NSCLC.Diagnostics (Basel, Switzerland) · 2021Article
- Genome analysis of peeling archival cytology samples detects driver mutations in lung cancer.Cancer medicine · 2020Article
- Multi-regional sequencing reveals clonal and polyclonal seeding from primary tumor to metastases in advanced gastric cancer.Journal of gastroenterology · 2020Article
- Dual-molecular barcode sequencing detects rare variants in tumor and cell free DNA in plasma.Scientific reports · 2020Article
- Genomic Profiling IdentifiedFrontiers in oncology · 2020Article
- Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR.BMC medical genomics · 2019Article
- Genomic profile of urine has high diagnostic sensitivity compared to cytology in non-invasive urothelial bladder cancer.Cancer science · 2019Article
- Molecular subtype switching in early-stage gastric cancers with multiple occurrences.Journal of gastroenterology · 2019Article
- Clinical Implications of Noncoding Indels in the Surfactant-Encoding Genes in Lung Cancer.Cancers · 2019Article
- Deficiency of mismatch repair genes is less frequently observed in signet ring cell compared with non-signet ring cell gastric cancer.Medical oncology (Northwood, London, England) · 2019Article
- Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics.Virchows Archiv : an international journal of pathology · 2018Article
- Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology.Journal of visualized experiments : JoVE · 2018Article
- New therapeutic targets for pulmonary sarcomatoid carcinomas based on their genomic and phylogenetic profiles.Oncotarget · 2018Article
- Next generation sequencing-based emerging trends in molecular biology of gastric cancer.American journal of cancer research · 2018Review
- Simultaneous detection of genetic and copy number alterations inOncotarget · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSequencing data from The Cancer Genome Atlas (TGCA), the International Cancer Genome Consortium and other research institutes have revealed the presence of genetic alterations in several tumor types, including gastric cancer. These data have been combined into a catalog of significantly mutated genes for each cancer type. However, it is unclear to what extent significantly mutated genes need to be examined for detecting genetic alterations in gastric cancer patients. Here, we constructed two custom-made sequencing panels of different scales, the Selective hotspot Panel and the Comprehensive Panel, to analyze genetic alterations in 21 resected specimens endoscopically obtained from 20 gastric cancer patients, and we assessed how many mutations were detectable using these different panels.
resultsA total of 21 somatic mutations were identified by the Selective hotspot Panel and 70 mutations were detected by the Comprehensive Panel. All mutations identified by the Selective hotspot Panel were detected by the Comprehensive Panel, with high concordant values of the variant allelic fraction of each mutation (correlation coefficient, R = 0.92). At least one mutation was identified in 13 patients (65 %) by the Selective hotspot Panel, whereas the Comprehensive Panel detected mutations in 19 (95 %) patients. Library preparation and sequencing costs were comparable between the two panels.
conclusionsOur results indicate the utility of comprehensive panel-based targeted sequencing in gastric cancer.
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