ArticleThe Review of scientific instruments2016
Surface plasmon resonance microscopy: Achieving a quantitative optical response.
Article in The Review of scientific instruments, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers.Chemical & biomedical imaging · 2026Review
- Metal-Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Improved imaging and preservation of lysosome dynamics using silver nanoparticle-enhanced fluorescence.Molecular biology of the cell · 2023Article
- In Situ Analysis of Membrane-Protein Binding Kinetics and Cell-Surface Adhesion Using Plasmonic Scattering Microscopy.Angewandte Chemie (International ed. in English) · 2022Article
- Exosome Processing and Characterization Approaches for Research and Technology Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Practical application of microsphere samples for benchmarking a quantitative phase imaging system.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Deep learning-based single-shot phase retrieval algorithm for surface plasmon resonance microscope based refractive index sensing application.Scientific reports · 2021Article
- Recent Advances in Surface Plasmon Resonance Imaging Sensors.Sensors (Basel, Switzerland) · 2019Review
- Mass Measurements of Focal Adhesions in Single Cells Using High Resolution Surface Plasmon Resonance Microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Surface plasmon resonance (SPR) imaging allows real-time label-free imaging based on index of refraction and changes in index of refraction at an interface. Optical parameter analysis is achieved by application of the Fresnel model to SPR data typically taken by an instrument in a prism based figuration. We carry out SPR imaging on a microscope by launching light into a sample and collecting reflected light through a high numerical aperture microscope objective. The SPR microscope enables spatial resolution that approaches the diffraction limit and has a dynamic range that allows detection of subnanometer to submicrometer changes in thickness of biological material at a surface. However, unambiguous quantitative interpretation of SPR changes using the microscope system could not be achieved using the Fresnel model because of polarization dependent attenuation and optical aberration that occurs in the high numerical aperture objective. To overcome this problem, we demonstrate a model to correct for polarization diattenuation and optical aberrations in the SPR data and develop a procedure to calibrate reflectivity to index of refraction values. The calibration and correction strategy for quantitative analysis was validated by comparing the known indices of refraction of bulk materials with corrected SPR data interpreted with the Fresnel model. Subsequently, we applied our SPR microscopy method to evaluate the index of refraction for a series of polymer microspheres in aqueous media and validated the quality of the measurement with quantitative phase microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.