ArticleHeredity2017
De novo transcriptome assembly facilitates characterisation of fast-evolving gene families, MHC class I in the bank vole (Myodes glareolus).
Article in Heredity, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Immunological surveillance against cancer across mammals.Nature communications · 2025Article
- Beyond the laboratory: the bank vole (Clethrionomys glareolus) as a novel model organism in biological research.Frontiers in zoology · 2025Review
- Bank vole genomics links determinate and indeterminate growth of teeth.BMC genomics · 2024Article
- Widespread Adaptive Introgression of Major Histocompatibility Complex Genes across Vertebrate Hybrid Zones.Molecular biology and evolution · 2024Article
- Vole genomics links determinate and indeterminate growth of teeth.bioRxiv : the preprint server for biology · 2024Article
- AmpliSAS and AmpliHLA: Web Server and Local Tools for MHC Typing of Non-model Species and Human Using NGS Data.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Evolutionary trade-offs constraining the MHC gene expansion: beyond simple TCR depletion model.Frontiers in immunology · 2023Article
- Characterization of two MHC II genes (DOB, DRB) in white-tailed deer (Odocoileus virginianus).BMC genetics · 2020Article
- Mating preferences can drive expansion or contraction of major histocompatibility complex gene family.Proceedings. Biological sciences · 2020Article
- Evolution of major histocompatibility complex gene copy number.PLoS computational biology · 2019Article
- Major histocompatibility complex class I diversity limits the repertoire of T cell receptors.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Preliminary insights into the genetics of bank vole tolerance to Puumala hantavirus in Sweden.Ecology and evolution · 2018Article
- Profiling of the TCRβ repertoire in non-model species using high-throughput sequencing.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major histocompatibility complex (MHC) plays a central role in the adaptive immune response and is the most polymorphic gene family in vertebrates. Although high-throughput sequencing has increasingly been used for genotyping families of co-amplifying MHC genes, its potential to facilitate early steps in the characterisation of MHC variation in nonmodel organism has not been fully explored. In this study we evaluated the usefulness of de novo transcriptome assembly in characterisation of MHC sequence diversity. We found that although de novo transcriptome assembly of MHC I genes does not reconstruct sequences of individual alleles, it does allow the identification of conserved regions for PCR primer design. Using the newly designed primers, we characterised MHC I sequences in the bank vole. Phylogenetic analysis of the partial MHC I coding sequence (2-4 exons) of the bank vole revealed a lack of orthology to MHC I of other Cricetidae, consistent with the high gene turnover of this region. The diversity of expressed alleles was characterised using ultra-deep sequencing of the third exon that codes for the peptide-binding region of the MHC molecule. High allelic diversity was demonstrated, with 72 alleles found in 29 individuals. Interindividual variation in the number of expressed loci was found, with the number of alleles per individual ranging from 5 to 14. Strong signatures of positive selection were found for 8 amino acid sites, most of which are inferred to bind antigens in human MHC, indicating conservation of structure despite rapid sequence evolution.
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