ArticleNeurogastroenterology and motility2017
Tumor necrosis factor alpha derived from classically activated "M1" macrophages reduces interstitial cell of Cajal numbers.
Article in Neurogastroenterology and motility, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
- Changes in interstitial cells of Cajal calcium activity in a diabetic mouse model of delayed gastric emptying.The Journal of physiology · 2026Article
- [Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026Article
- Mass cytometric analysis of circulating monocyte subsets in a murine model of diabetic gastroparesis.American journal of physiology. Gastrointestinal and liver physiology · 2025Article
- Emerging roles of traditional Chinese medicine in the treatment of diabetic gastroparesis.Frontiers in nutrition · 2025Review
- Cancer marker TNFRSF1A: From single‑cell heterogeneity of renal cell carcinoma to functional validation.Oncology letters · 2024Article
- Single cell atlas of human gastric muscle immune cells and macrophage-driven changes in idiopathic gastroparesis.iScience · 2024Article
- The critical role of muscularis macrophages in modulating the enteric nervous system function and gastrointestinal motility.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2024Review
- The enteric nervous system.Physiological reviews · 2023Review
- Systemic Cytokine Expression in Diabetes Is Associated with Prolonged Gastrointestinal Transit Times and Cardinal Gastroparesis Symptoms.Biomedicines · 2023Article
- Interstitial cells of Cajal in gastrointestinal inflammatory diseases.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2023Review
- The Role of Inflammatory Mediators in the Development of Gastrointestinal Motility Disorders.International journal of molecular sciences · 2022Review
- Role of Macrophages and Mast Cells as Key Players in the Maintenance of Gastrointestinal Smooth Muscle Homeostasis and Disease.Cellular and molecular gastroenterology and hepatology · 2022Review
- Protective Effect of Tangshen Formula () on Interstitial Cells of Cajal in Colon of Diabetic Rats.Chinese journal of integrative medicine · 2022Article
- Intelligent H2S release coating for regulating vascular remodeling.Bioactive materials · 2021Article
- Intestinal dysmotility after bowel resection in rats is associated with decreased ghrelin and vimentin expression and loss of intestinal cells of Cajal.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Intestinal proinflammatory macrophages induce a phenotypic switch in interstitial cells of Cajal.The Journal of clinical investigation · 2020Article
- Gastric Biopsies in Gastroparesis: Insights into Gastric Neuromuscular Disorders to Aid Treatment.Gastroenterology clinics of North America · 2020Review
- Proteomics in gastroparesis: unique and overlapping protein signatures in diabetic and idiopathic gastroparesis.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis.Oxidative medicine and cellular longevity · 2019Article
- The phenotypic and functional properties of mouse yolk-sac-derived embryonic macrophages.Developmental biology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundDelayed gastric emptying in diabetic mice and humans is associated with changes in macrophage phenotype and loss of interstitial cells of Cajal (ICC) in the gastric muscle layers. In diabetic mice, classically activated M1 macrophages are associated with delayed gastric emptying, whereas alternatively activated M2 macrophages are associated with normal gastric emptying. This study aimed to determine if secreted factors from M1 macrophages could injure mouse ICC in primary culture.
methodsCultures of gastric ICC were treated with conditioned medium (CM) from activated bone marrow-derived macrophages (BMDMs) and the effect of CM was quantified by counting ICC per high-powered field. KEY
resultsBone marrow-derived macrophages were activated to a M1 or M2 phenotype confirmed by qRT-PCR. Conditioned medium from M1 macrophages reduced ICC numbers by 41.1%, whereas M2-CM had no effect as compared to unconditioned, control media. Immunoblot analysis of 40 chemokines/cytokines found 12 that were significantly increased in M1-CM, including tumor necrosis factor alpha (TNF-α). ELISA detected 0.697±0.03 ng mL CONCLUSIONS & INFERENCES: This work demonstrates that TNF-α secreted from M1 macrophages can result in Kit loss and directly injure ICC in vitro partly through caspase-dependent apoptosis and may play an important role in ICC depletion in diabetic gastroparesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.