ArticleThe Journal of biological chemistry2016
Human Metabolome-derived Cofactors Are Required for the Antibacterial Activity of Siderocalin in Urine.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Cutibacterium Adaptation to Life on Humans Provides a Potential C acnes Infection Biomarker.The Journal of investigative dermatology · 2026Article
- In defence of ferroptosis.Signal transduction and targeted therapy · 2025Review
- Article
- Serum Lipocalin-2 Levels as a Biomarker in Pre- and Post-Pubertal Klinefelter Syndrome Patients: A Pilot Study.International journal of molecular sciences · 2024Article
- Regulation of the innate immune response and gut microbiome by p53.Cancer science · 2024Article
- A Differential Protein Study on Bronchoalveolar Lavage Fluid at Different Stages of Silicosis.Combinatorial chemistry & high throughput screening · 2024Article
- Uropathogenic Escherichia coli wield enterobactin-derived catabolites as siderophores.The Journal of biological chemistry · 2024Article
- UropathogenicbioRxiv : the preprint server for biology · 2023Article
- Iron Status and Supplementation during Tuberculosis.Microorganisms · 2023Review
- Mycobiota and diet-derived fungal xenosiderophores promote Salmonella gastrointestinal colonization.Nature microbiology · 2022Article
- ThemBio · 2022Article
- Innate Bacteriostatic Mechanisms Defend the Urinary Tract.Annual review of physiology · 2022Review
- Mass Spectrometry-Based Structural Proteomics for Metal Ion/Protein Binding Studies.Biomolecules · 2022Review
- Systematic analysis of myocardial immune progression in septic cardiomyopathy: Immune-related mechanisms in septic cardiomyopathy.Frontiers in cardiovascular medicine · 2022Article
- The effect of lipocalin-2 (LCN2) on apoptosis: a proteomics analysis study in an LCN2 deficient mouse model.BMC genomics · 2021Article
- Lipocalin-2: a role in hepatic gluconeogenesis via AMP-activated protein kinase (AMPK).Journal of endocrinological investigation · 2021Article
- The Underlying Molecular Mechanisms Involved in Traditional Chinese MedicineEvidence-based complementary and alternative medicine : eCAM · 2021Article
- Protein profile of well-differentiated versus un-differentiated human bronchial/tracheal epithelial cells.Heliyon · 2020Article
- Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry.ACS chemical biology · 2020Article
- Crystal Structure of the Siderophore Binding Protein BauB Bound to an Unusual 2:1 Complex Between Acinetobactin and Ferric Iron.Biochemistry · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
In human urinary tract infections, host cells release the antimicrobial protein siderocalin (SCN; also known as lipocalin-2, neutrophil gelatinase-associated lipocalin, or 24p3) into the urinary tract. By binding to ferric catechol complexes, SCN can sequester iron, a growth-limiting nutrient for most bacterial pathogens. Recent evidence links the antibacterial activity of SCN in human urine to iron sequestration and metabolomic variation between individuals. To determine whether these metabolomic associations correspond to functional Fe(III)-binding SCN ligands, we devised a biophysical protein binding screen to identify SCN ligands through direct analysis of human urine. This screen revealed a series of physiologic unconjugated urinary catechols that were able to function as SCN ligands of which pyrogallol in particular was positively associated with high urinary SCN activity. In a purified, defined culture system, these physiologic SCN ligands were sufficient to activate SCN antibacterial activity against Escherichia coli In the presence of multiple SCN ligands, native mass spectrometry demonstrated that SCN may preferentially combine different ligands to coordinate iron, suggesting that availability of specific ligand combinations affects in vivo SCN antibacterial activity. These results support a mechanistic link between the human urinary metabolome and innate immune function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.