Evidence map›Paper›PMID 27780544›Full record

ArticlePlacenta2016

Isolation of human trophoblastic extracellular vesicles and characterization of their cargo and antiviral activity.

Yingshi Ouyang, Avraham Bayer, Tianjiao Chu, Vladimir A Tyurin, Valerian E Kagan, Adrian E Morelli, Carolyn B Coyne, Yoel Sadovsky

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Placenta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
4.0field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 102 citations in OpenAlex.

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  8. The immune-modulatory dynamics of exosomes in preeclampsia.Archives of gynecology and obstetrics · 2025
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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Yingshi OuyangMagee-Womens Research Institute, Department of OBGYN and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Avraham BayerMagee-Womens Research Institute, Department of OBGYN and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Tianjiao ChuMagee-Womens Research Institute, Department of OBGYN and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Vladimir A TyurinDepartment of Environmental and Occupational Health, Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Valerian E KaganDepartment of Environmental and Occupational Health, Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, PA 15219, USA.
Adrian E MorelliT.E. Starzl Institute and Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Carolyn B CoyneDepartment of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA. Electronic address: coynec2@pitt.edu.
Yoel SadovskyMagee-Womens Research Institute, Department of OBGYN and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15219, USA. Electronic address: ysadovsky@mwri.magee.edu.
University of Pittsburgh · US

Funding

VECTOR CORE FACILITYP30CA047904 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Leonard J. Appleman · 1988 to 2026
$158.0M
Transcriptional and developmental regulation of placental fuel storage and metabP01HD069316 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI BARAK, YAACOV · 2012 to 2016
$5.0M
Enterovirus Infection of Polarized Intestinal CellsR01AI081759 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI COYNE, CAROLYN B · 2009 to 2024
$5.0M
Extracellular vesicles and their ncRNA cargo as markers of trophoblast injuryR01HD086325 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI SADOVSKY, YOEL · 2015 to 2019
$3.6M
Primary human trophoblasts and the transfer of viral resistanceR01HD075665 · NICHD · MAGEE-WOMEN'S RES INST AND FOUNDATION · PI COYNE, CAROLYN B, SADOVSKY, YOEL · 2012 to 2016
$2.4M
NCI NIH HHS P30 CA047904NIAID NIH HHS R01 AI081759NICHD NIH HHS P01 HD069316NICHD NIH HHS R01 HD075665NICHD NIH HHS R01 HD086325Wellcome Trust
6 · The paper itself

Abstract

introductionPrimary human trophoblasts release a repertoire of extracellular vesicles (EVs). Among them are nano-sized exosomes, which we found to suppress the replication of a wide range of diverse viruses. These exosomes contain trophoblastic microRNAs (miRNAs) that are expressed from the chromosome 19 miRNA cluster and exhibit antiviral properties. Here, we report our investigation of the cargo of placental EVs, focusing on the composition and the antiviral properties of exosomes, microvesicles, and apoptotic blebs.

methodsWe isolated EVs using ultracentrifugation and defined their purity using immunoblotting, electron microscopy, and nanoparticle tracking. We used liquid chromatography-electrospray ionization-mass spectrometry, protein mass spectrometry, and miRNA TaqMan card PCR to examine the phospholipids, proteins, and miRNA cargo of trophoblastic EVs and an in vitro viral infection assay to assess the antiviral properties of EVs.

resultsWe found that all three EV types contain a comparable repertoire of miRNA. Interestingly, trophoblastic exosomes harbor a protein and phospholipid profile that is distinct from that of microvesicles or apoptotic blebs. Functionally, trophoblastic exosomes exhibit the highest antiviral activity among the EVs. Consistently, plasma exosomes derived from pregnant women recapitulate the antiviral effect of trophoblastic exosomes derived from in vitro cultures of primary human trophoblasts. DISCUSSION: When compared to other trophoblastic EVs, exosomes exhibit a unique repertoire of proteins and phospholipids, but not miRNAs, and a potent viral activity. Our work suggests that human trophoblastic EVs may play a key role in maternal-placental-fetal communication.

Indexed as

ExosomesExtracellular VesiclesFemaleHumansMass SpectrometryMicroRNAsPhospholipidsPlacentaPregnancyTrophoblastsMicroRNAsPhospholipidsC19MCExosomesPlacentaPlasma exosomesTrophoblastic vesiclesViral infection

Identifiers

PMID27780544
PMCPMC5123854
OpenAlexW2519310617

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.