Evidence map›Paper›PMID 27778278›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.

Yu Qian, Eranthie Weerapana

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Flavonoids in Ampelopsis grossedentata as covalent inhibitors of SARS-CoV-2 3CLInternational journal of biological macromolecules · 2021
    Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Reactive-cysteine profiling for drug discovery.Current opinion in chemical biology · 2019
    Review
  7. Article
  8. Arylation Chemistry for Bioconjugation.Angewandte Chemie (International ed. in English) · 2019
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yu QianDepartment of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, USA.
Eranthie WeerapanaDepartment of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA, USA. eranthie@bc.edu.

Funding

Investigating cysteine-mediated protein activities in C. elegansR01GM118431 · NIGMS · BOSTON COLLEGE · PI WEERAPANA, ERANTHIE · 2015 to 2019
$1.8M
Investigating cysteine PTMs in living cellsR01GM117004 · NIGMS · BOSTON COLLEGE · PI WEERAPANA, ERANTHIE · 2016 to 2019
$1.2M
NIGMS NIH HHS R01 GM117004NIGMS NIH HHS R01 GM118431
6 · The paper itself

Abstract

Cysteine residues on proteins serve diverse functional roles in catalysis and regulation and are susceptible to numerous posttranslational modifications. Methods to monitor the reactivity of cysteines within the context of a complex proteome have facilitated the identification and functional characterization of cysteine residues on disparate proteins. Here, we describe the use of a cysteine-reactive iodoacetamide probe coupled to isotopically labeled, cleavable linkers to identify and quantify cysteine-reactivity changes from two biological samples.

Indexed as

AnimalsCysteineIodoacetamideMass SpectrometryProtein Processing, Post-TranslationalProteomeCysteineIodoacetamideProteomeChemically cleavable azobenzene linkerCu + clickCysteine-reactivityIodoacetamide-alkyneisoTOP-ABPPMudPIT

Identifiers

PMID27778278
PMCPMC5359601

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.