Evidence map›Paper›PMID 27777610›Full record

ArticlePlant methods2016

Infiltration-RNAseq: transcriptome profiling of Agrobacterium-mediated infiltration of transcription factors to discover gene function and expression networks in plants.

Donna M Bond, Nick W Albert, Robyn H Lee, Gareth B Gillard, Chris M Brown, Roger P Hellens, Richard C Macknight

Open access · goldAbstract read
In one paragraph

Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 44 citations in OpenAlex.

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  15. An efficient system forPlant methods · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Donna M BondDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, 9054 New Zealand.
Nick W AlbertThe New Zealand Institute for Plant and Food Research Limited, Private Bag 11-600, Palmerston North, New Zealand.
Robyn H LeeDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, 9054 New Zealand.
Gareth B GillardDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, 9054 New Zealand ; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1432 Ås, Norway.
Chris M BrownDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, 9054 New Zealand.
Roger P HellensCentre for Tropical Crops and Biocommodities, Institute for Future Environments, Queensland University of Technology, GPO Box 2434, Brisbane, QLD 4001 Australia.
Richard C MacknightDepartment of Biochemistry, University of Otago, PO Box 56, Dunedin, 9054 New Zealand ; The New Zealand Institute for Plant and Food Research Limited, Private Bag 11-600, Palmerston North, New Zealand.
University of Otago · NZPlant & Food Research · NZQueensland University of Technology · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTranscription factors (TFs) coordinate precise gene expression patterns that give rise to distinct phenotypic outputs. The identification of genes and transcriptional networks regulated by a TF often requires stable transformation and expression changes in plant cells. However, the production of stable transformants can be slow and laborious with no guarantee of success. Furthermore, transgenic plants overexpressing a TF of interest can present pleiotropic phenotypes and/or result in a high number of indirect gene expression changes. Therefore, fast, efficient, high-throughput methods for assaying TF function are needed.

resultsAgroinfiltration is a simple plant biology method that allows transient gene expression. It is a rapid and powerful tool for the functional characterisation of TF genes

conclusionsInfiltration-RNAseq is a fast and convenient method for profiling TF-mediated gene expression changes. We utilised this method to identify TF-mediated transcriptional changes and TF target genes in

Indexed as

Agrobacterium tumefaciensAnthocyaninInfiltrationMedicago truncatulaMtLAP1Nicotiana benthamianaRNAseqTranscription factorTranscriptome

Identifiers

PMID27777610
PMCPMC5069895
OpenAlexW2532518305

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.