ArticleRNA (New York, N.Y.)2017
Artificial "ping-pong" cascade of PIWI-interacting RNA in silkworm cells.
Article in RNA (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Non-gonadal somatic piRNA pathways ensure sexual differentiation, larval growth, and wing development in silkworms.PLoS genetics · 2023Article
- The piRNA cluster torimochi is an expanding transposon in cultured silkworm cells.PLoS genetics · 2023Article
- RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors.Nature communications · 2021Article
- DEAD-box polypeptide 43 facilitates piRNA amplification by actively liberating RNA from Ago3-piRISC.EMBO reports · 2021Article
- Article
- Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis.Nature · 2018Article
- Transcriptome profiling reveals infection strategy of an insect maculavirus.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
PIWI-interacting RNAs (piRNAs) play essential roles in the defense system against selfish elements in animal germline cells by cooperating with PIWI proteins. A subset of piRNAs is predicted to be generated via the "ping-pong" cascade, which is mainly controlled by two different PIWI proteins. Here we established a cell-based artificial piRNA production system using a silkworm ovarian cultured cell line that is believed to possess a complete piRNA pathway. In addition, we took advantage of a unique silkworm sex-determining one-to-one ping-pong piRNA pair, which enabled us to precisely monitor the behavior of individual artificial piRNAs. With this novel strategy, we successfully generated artificial piRNAs against endogenous protein-coding genes via the expected back-and-forth traveling mechanism. Furthermore, we detected "primary" piRNAs from the upstream region of the artificial "ping-pong" site in the endogenous gene. This artificial piRNA production system experimentally confirms the existence of the "ping-pong" cascade of piRNAs. Also, this system will enable us to identify the factors involved in both, or each, of the "ping" and "pong" cascades and the sequence features that are required for efficient piRNA production.
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