ArticlePlant biotechnology journal2017
Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK-6.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Benzenoid Aromatics from Renewable Resources.Chemical reviews · 2024Review
- ZnO nanoparticles encapsulated cellulose-lignin film for antibacterial and biodegradable food packaging.iScience · 2024Article
- Utilising Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) to track the oxidation of lignin by an alkaliphilic laccase.The Analyst · 2024Article
- Transcriptomic and metabolomic analysis reveals the influence of carbohydrates on lignin degradation mediated byFrontiers in microbiology · 2024Article
- A nostoxanthin-producing bacterium,Frontiers in microbiology · 2023Article
- Lignocellulose dissociation with biological pretreatment towards the biochemical platform: A review.Materials today. Bio · 2022Review
- Engineering Curcumin Biosynthesis in Poplar Affects Lignification and Biomass Yield.Frontiers in plant science · 2022Article
- Spatio-Temporal Modification of Lignin Biosynthesis in Plants: A Promising Strategy for Lignocellulose Improvement and Lignin Valorization.Frontiers in bioengineering and biotechnology · 2022Review
- A plant host, Nicotiana benthamiana, enables the production and study of fungal lignin-degrading enzymes.Communications biology · 2021Article
- Enhanced lignin biodegradation by consortium of white rot fungi: microbial synergistic effects and product mapping.Biotechnology for biofuels · 2021Article
- Depolymerization and conversion of lignin to value-added bioproducts by microbial and enzymatic catalysis.Biotechnology for biofuels · 2021Review
- Applicability of Recombinant Laccases From the White-Rot FungusFrontiers in bioengineering and biotechnology · 2020Article
- Review
- Lignin valorization meets synthetic biology.Engineering in life sciences · 2019Review
- RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family.Plant biotechnology journal · 2019Article
- Introducing curcumin biosynthesis in Arabidopsis enhances lignocellulosic biomass processing.Nature plants · 2019Article
- Biodegradation of lignin by Pseudomonas sp. Q18 and the characterization of a novel bacterial DyP-type peroxidase.Journal of industrial microbiology & biotechnology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the β-aryl ether bond. In this study, we expressed the LigD, LigF and LigG (LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized β-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.