Evidence map›Paper›PMID 27775603›Full record

ArticleInternational journal of molecular sciences2016

Zinc Finger and X-Linked Factor (ZFX) Binds to Human SET Transcript 2 Promoter and Transactivates SET Expression.

Siliang Xu, Ping Duan, Jinping Li, Tristan Senkowski, Fengbiao Guo, Haibin Chen, Alberto Romero, Yugui Cui, Jiayin Liu, Shi-Wen Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Development and identification of Set transgenic mice.Experimental and therapeutic medicine · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Siliang XuThe State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. xu_s@mercer.edu.
Ping DuanDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China. cuiygnj@njmu.edu.cn.
Jinping LiDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. li_j@mercer.edu.
Tristan SenkowskiDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. senkowskitristan@savcds.org.
Fengbiao GuoDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. guo_f@mercer.edu.
Haibin ChenDepartment of Histology and Embryology, Shantou University Medical College, Shantou 515000, China. chenhb@stu.edu.cn.
Alberto RomeroDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. romero_ah@med.mercer.edu.
Yugui CuiThe State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. cuiygnj@njmu.edu.cn.
Jiayin LiuThe State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China. jyliu_nj@njmu.edu.cn.
Shi-Wen JiangDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31404, USA. jiang_s@mercer.edu.
Mercer University · USNanjing Medical University · CNShantou University Medical College · CNWenzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

SET (SE Translocation) protein carries out multiple functions including those for protein phosphatase 2A (PP2A) inhibition, histone modification, DNA repair, and gene regulation. SET overexpression has been detected in brain neurons of patients suffering Alzheimer's disease, follicle theca cells of Polycystic Ovary Syndrome (PCOS) patients, and ovarian cancer cells, indicating that SET may play a pathological role for these disorders. SET transcript 2, produced by a specific promoter, represents a major transcript variant in different cell types. In this study, we characterized the transcriptional activation of human SET transcript 2 promoter in HeLa cells. Promoter deletion experiments and co-transfection assays indicated that ZFX, the Zinc finger and X-linked transcription factor, was able to transactivate the SET promoter. A proximal promoter region containing four ZFX-binding sites was found to be critical for the ZFX-mediated transactivation. Mutagenesis study indicated that the ZFX-binding site located the closest to the transcription start site accounted for most of the ZFX-mediated transactivity. Manipulation of ZFX levels by overexpression or siRNA knockdown confirmed the significance and specificity of the ZFX-mediated SET promoter activation. Chromatin immunoprecipitation results verified the binding of ZFX to its cognate sites in the SET promoter. These findings have led to identification of ZFX as an upstream factor regulating

Indexed as

Base SequenceBinding SitesCell Line, TumorChromatin ImmunoprecipitationDNA-Binding ProteinsGene Expression RegulationHEK293 CellsHeLa CellsHistone ChaperonesHumansKruppel-Like Transcription FactorsPromoter Regions, GeneticProtein BindingRNA InterferenceRNA, Small InterferingTranscriptional ActivationDNA-Binding ProteinsHistone ChaperonesKruppel-Like Transcription FactorsRNA, Small InterferingSET protein, humanTranscription Factorszinc finger protein, X-linkedgynecologic cancersI2PP2A (protein phosphatase 2A inhibitor)SET (SE Translocation)transcriptional regulationZFX (Zinc finger and X-linked transcription factor)

Identifiers

PMID27775603
PMCPMC5085766
OpenAlexW2529506885

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.