Evidence map›Paper›PMID 27775041›Full record

ArticleScientific reports2016

Stretching human mesenchymal stromal cells on stiffness-customized collagen type I generates a smooth muscle marker profile without growth factor addition.

Miriam Rothdiener, Miriam Hegemann, Tatiana Uynuk-Ool, Brandan Walters, Piruntha Papugy, Phong Nguyen, Valentin Claus, Tanja Seeger, Ulrich Stoeckle, Karen A Boehme and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 31 citations in OpenAlex.

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  12. Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization.Journal of tissue engineering and regenerative medicine · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Miriam RothdienerSiegfried Weller Institute for Trauma Research, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Miriam HegemannDepartment of Urology, University of Tuebingen, Germany.
Tatiana Uynuk-OolSiegfried Weller Institute for Trauma Research, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Brandan WaltersDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Piruntha PapugySiegfried Weller Institute for Trauma Research, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Phong NguyenSiegfried Weller Institute for Trauma Research, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Valentin ClausSiegfried Weller Institute for Trauma Research, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Tanja SeegerCenter for Medical Research, Medical University Clinic II, University of Tuebingen, Germany.
Ulrich StoeckleClinic for Trauma and Restorative Surgery, BG Trauma Clinic Tuebingen, University of Tuebingen, Germany.
Karen A BoehmeDepartment of Orthopaedic Surgery, University of Tuebingen, Germany.
Wilhelm K AicherDepartment of Urology, University of Tuebingen, Germany.
Jan P StegemannDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Melanie L HartDepartment of Orthopedics and Trauma Surgery, Albert-Ludwigs-University, Freiburg, Germany.
Bodo KurzDepartment of Anatomy, Christian-Albrechts-University, Kiel, Germany.
Gerd KleinCenter for Medical Research, Medical University Clinic II, University of Tuebingen, Germany.
Bernd RolauffsDepartment of Orthopedics and Trauma Surgery, Albert-Ludwigs-University, Freiburg, Germany.
University of Tübingen · DEUniversity of Freiburg · DEUniversity of Michigan–Ann Arbor · USKiel University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using matrix elasticity and cyclic stretch have been investigated for inducing mesenchymal stromal cell (MSC) differentiation towards the smooth muscle cell (SMC) lineage but not in combination. We hypothesized that combining lineage-specific stiffness with cyclic stretch would result in a significantly increased expression of SMC markers, compared to non-stretched controls. First, we generated dense collagen type I sheets by mechanically compressing collagen hydrogels. Atomic force microscopy revealed a nanoscale stiffness range known to support myogenic differentiation. Further characterization revealed viscoelasticity and stable biomechanical properties under cyclic stretch with >99% viable adherent human MSC. MSCs on collagen sheets demonstrated a significantly increased mRNA but not protein expression of SMC markers, compared to on culture flasks. However, cyclic stretch of MSCs on collagen sheets significantly increased both mRNA and protein expression of α-smooth muscle actin, transgelin, and calponin versus plastic and non-stretched sheets. Thus, lineage-specific stiffness and cyclic stretch can be applied together for inducing MSC differentiation towards SMCs without the addition of recombinant growth factors or other soluble factors. This represents a novel stimulation method for modulating the phenotype of MSCs towards SMCs that could easily be incorporated into currently available methodologies to obtain a more targeted control of MSC phenotype.

Indexed as

ActinsBiomarkersBone Marrow CellsCalcium-Binding ProteinsCalponinsCell Culture TechniquesCell DifferentiationCells, CulturedCollagen Type IGene Expression RegulationHumansMesenchymal Stem CellsMicrofilament ProteinsMicroscopy, Atomic ForceMuscle ProteinsMuscle, SmoothACTA2 protein, humanActinsBiomarkersCalcium-Binding ProteinsCalponinsCollagen Type IMicrofilament ProteinsMuscle ProteinstransgelinTubulin

Identifiers

PMID27775041
PMCPMC5075785
OpenAlexW2537390755

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.