ArticleExperimental neurology2017
Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome.
Article in Experimental neurology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Gene expression in stress urinary incontinence: a systematic review.International urogynecology journal · 2020Pooled it
- Article
- Diagnostic Criteria and Technical Evaluation of Complex Regional Pain Syndrome: A Narrative Review.Diagnostics (Basel, Switzerland) · 2025Review
- Modeling Neuropathic Corneal Pain: Pulled Nerve Approach With Elevated Krt16 Gene Expression.Investigative ophthalmology & visual science · 2025Article
- Current and Evolving Concepts in the Management of Complex Regional Pain Syndrome: A Narrative Review.Diagnostics (Basel, Switzerland) · 2025Review
- Alpha-7 Nicotinic Acetylcholine Receptor Activation Inhibits Trauma Induced Pronociceptive Autoimmune Responses.The journal of pain · 2024Article
- Transient immune activation without loss of intraepidermal innervation and associated Schwann cells in patients with complex regional pain syndrome.Journal of neuroinflammation · 2024Article
- Rapamycin Augmentation of Chronic Ketamine as a Novel Treatment for Complex Regional Pain Syndrome.Cureus · 2023Article
- Cerebrospinal Fluid Protein Biomarker Discovery in CLN3.Journal of proteome research · 2023Article
- The autoimmune aetiology of unexplained chronic pain.Autoimmunity reviews · 2022Review
- Autonomic Regulation of Nociceptive and Immunologic Changes in a Mouse Model of Complex Regional Pain Syndrome.The journal of pain · 2022Article
- IL-6 signaling mediates the germinal center response, IgM production and nociceptive sensitization in male mice after tibia fracture.Brain, behavior, and immunity · 2021Article
- Autoimmune regulation of chronic pain.Pain reports · 2021Review
- Article
- DNA methylation profiles are associated with complex regional pain syndrome after traumatic injury.Pain · 2019Article
- Expansion and activation of distinct central memory T lymphocyte subsets in complex regional pain syndrome.Journal of neuroinflammation · 2019Article
- The Rodent Tibia Fracture Model: A Critical Review and Comparison With the Complex Regional Pain Syndrome Literature.The journal of pain · 2018Review
- Neuropeptide regulation of adaptive immunity in the tibia fracture model of complex regional pain syndrome.Journal of neuroinflammation · 2018Article
- Article
- Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveUsing a mouse model of complex regional pain syndrome (CRPS), our goal was to identify autoantigens in the skin of the affected limb.
methodsA CRPS-like state was induced using the tibia fracture/cast immobilization model. Three weeks after fracture, hindpaw skin was homogenized, run on 2-d gels, and probed by sera from fracture and control mice. Spots of interest were analyzed by liquid chromatography-mass spectroscopy (LC-MS) and the list of targets validated by examining their abundance and subcellular localization. In order to measure the autoantigenicity of selected protein targets, we quantified the binding of IgM in control and fracture mice sera, as well as in control and CRPS human sera, to the recombinant protein.
resultsWe show unique binding between fracture skin extracts and fracture sera, suggesting the presence of auto-antigens. LC-MS analysis provided us a list of potential targets, some of which were upregulated after fracture (KRT16, eEF1a1, and PRPH), while others showed subcellular-redistribution and increased membrane localization (ANXA2 and ENO3). No changes in protein citrullination or carbamylation were observed. In addition to increased abundance, KRT16 demonstrated autoantigenicity, since sera from both fracture mice and CRPS patients showed increased autoantibody binding to recombinant kRT16 protein.
conclusionsPursuing autoimmune contributions to CRPS provides a novel approach to understanding the condition and may allow the development of mechanism-based therapies. The identification of autoantibodies against KRT16 as a biomarker in mice and in humans is a critical step towards these goals, and towards redefining CRPS as having an autoimmune etiology.
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Registered trials
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