ArticleVirology2017
In vitro exposure system for study of aerosolized influenza virus.
Article in Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- An Inverse Transwell Assay for Airway Mucus Barrier Function Reveals both Virus- and Mucin-Specific Impacts on Infection.bioRxiv : the preprint server for biology · 2026Article
- Air-liquid interface model for influenza aerosol exposureJournal of virology · 2025Article
- A New Sensitive Sensor Test for Capturing and Evaluating Bacteria and Viruses in Airborne Aerosols.Sensors (Basel, Switzerland) · 2025Article
- Nasal Mucociliary Epithelial Cell Culture Models for Studying Viral Infections.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A Pilot Study of Aerosolization of Infectious Murine Norovirus in an Experimental Setup.Food and environmental virology · 2024Article
- Improvement of Sensitivity and Speed of Virus Sensing Technologies Using nm- and μm-Scale Components.Sensors (Basel, Switzerland) · 2023Review
- Inherent Heterogeneity of Influenza A Virus Stability following Aerosolization.Applied and environmental microbiology · 2022Article
- Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey.The Science of the total environment · 2021Article
- RNA oxidation in chromatin modification and DNA-damage response following exposure to formaldehyde.Scientific reports · 2020Article
- Ferreting Out Influenza Virus Pathogenicity and Transmissibility: Past and Future Risk Assessments in the Ferret Model.Cold Spring Harbor perspectives in medicine · 2020Review
- Cell culture keeps pace with influenza virus.The Lancet. Respiratory medicine · 2018Article
- Infection and Replication of Influenza Virus at the Ocular Surface.Journal of virology · 2018Article
- Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus.Methods in molecular biology (Clifton, N.J.) · 2018Article
- In Vitro Models to Study Human Lung Development, Disease and Homeostasis.Physiology (Bethesda, Md.) · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Infection of adherent cell monolayers using a liquid inoculum represents an established method to reliably and quantitatively study virus infection, but poorly recapitulates the exposure and infection of cells in the respiratory tract that occurs during infection with aerosolized pathogens. To better simulate natural infection in vitro, we adapted a system that generates viral aerosols similar to those exhaled by infected humans to the inoculation of epithelial cell monolayers. Procedures for cellular infection and calculation of exposure dose were developed and tested using viruses characterized by distinct transmission and pathogenicity phenotypes: an HPAI H5N1, an LPAI H7N9, and a seasonal H3N2 virus. While all three aerosolized viruses were highly infectious in a human bronchial epithelial cell line (Calu-3) cultured submerged in media, differences between the viruses were observed in primary human alveolar epithelial cells and in Calu-3 cells cultured at air-liquid interface. This system provides a novel enhancement to traditional in vitro experiments, particularly those focused on the early stages of infection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.