ArticleAnalytical biochemistry2017
Gaussian and linear deconvolution of LC-MS/MS chromatograms of the eight aminobutyric acid isomers.
Article in Analytical biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- An exploratory investigation of β-aminoisobutyric acid as a biomarker: possible relationships between urinary BAIBA and lifestyle habits.Biochemistry and biophysics reports · 2026Article
- Genome Mining Leads to the Discovery of Kasichelins A-D, Unusual β-Alanine- and β-Aminoisobutyric Acid-Containing Siderophores fromJournal of natural products · 2025Article
- Metabolomics: Uncovering Insights into Obesity and Diabetes.International journal of molecular sciences · 2025Article
- Quantum chemical study of molecular properties of small branched-chain amino acids in water.Amino acids · 2025Article
- Stereoselective Amine-omics Using Heavy Atom Isotope Labeled l- and d-Marfey's Reagents.Journal of the American Society for Mass Spectrometry · 2024Article
- An Automated Workflow Composition System for Liquid Chromatography-Mass Spectrometry Metabolomics Data Processing.Journal of the American Society for Mass Spectrometry · 2023Article
- Deconvolution of multichannel LC-MS/MS chromatograms of glucosamine-phosphates: Evidence of a GlmS regulatory difference betweenTalanta open · 2023Article
- A Novel Gliotransmitter, L-β-Aminoisobutyric Acid, Contributes to Pathophysiology of Clinical Efficacies and Adverse Reactions of Clozapine.Biomolecules · 2023Review
- High-Throughput Screening of Natural Product and Synthetic Molecule Libraries for Antibacterial Drug Discovery.Metabolites · 2023Review
- Simultaneous determination of all aminobutyric acids by chiral derivatization and liquid chromatography-tandem mass spectrometry.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Signaling metabolite β-aminoisobutyric acid as a metabolic regulator, biomarker, and potential exercise pill.Frontiers in endocrinology · 2023Review
- Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis.Angewandte Chemie (International ed. in English) · 2022Article
- β-Aminoisobutyric acid, L-BAIBA, protects PC12 cells from hydrogen peroxide-induced oxidative stress and apoptosis via activation of the AMPK and PI3K/Akt pathway.IBRO neuroscience reports · 2022Article
- Sphaerostilbellins, New Antimicrobial Aminolipopeptide Peptaibiotics fromBiomolecules · 2020Article
- Quantification of aminobutyric acids and their clinical applications as biomarkers for osteoporosis.Communications biology · 2020Article
- LC-MS/MS-Based Separation and Quantification of Marfey's Reagent Derivatized Proteinogenic Amino Acid DL-Stereoisomers.Journal of the American Society for Mass Spectrometry · 2019Article
- Beta-Aminoisobutyric Acid as a Novel Regulator of Carbohydrate and Lipid Metabolism.Nutrients · 2019Review
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5 authors.
Funding
Abstract
Isomeric molecules present a challenge for analytical resolution and quantification, even with MS-based detection. The eight aminobutyric acid (ABA) isomers are of interest for their various biological activities, particularly γ-aminobutyric acid (GABA) and the d- and l-isomers of β-aminoisobutyric acid (β-AIBA; BAIBA). This study aimed to investigate LC-MS/MS-based resolution of these ABA isomers as their Marfey's (Mar) reagent derivatives. HPLC was able to separate three Mar-ABA isomers l-β-ABA (l-BABA), and l- and d-α-ABA (AABA) completely, with three isomers (GABA, and d/l-BAIBA) in one chromatographic cluster, and two isomers (α-AIBA (AAIBA) and d-BABA) in a second cluster. Partially separated cluster components were deconvoluted using Gaussian peak fitting except for GABA and d-BAIBA. MS/MS detection of Marfey's derivatized ABA isomers provided six MS/MS fragments, with substantially different intensity profiles between structural isomers. This allowed linear deconvolution of ABA isomer peaks. Combining HPLC separation with linear and Gaussian deconvolution allowed resolution of all eight ABA isomers. Application to human serum found a substantial level of l-AABA (13 μM), an intermediate level of l-BAIBA (0.8 μM), and low but detectable levels (<0.2 μM) of GABA, l-BABA, AAIBA, d-BAIBA, and d-AABA. This approach should be useful for LC-MS/MS deconvolution of other challenging groups of isomeric molecules.
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