ArticleVeterinary microbiology2016
Selective medium for culture of Mycoplasma hyopneumoniae.
Article in Veterinary microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Comparative evaluation and validation of rapid quantification methods for Mycoplasma hyopneumoniae: development of a PMA-based viability qPCR assay.Scientific reports · 2026Article
- A cross-sectional study of keratoconjunctivitis among dairy cattle farms subject to Mediterranean climatic conditions.Tropical animal health and production · 2025Article
- TheFrontiers in cellular and infection microbiology · 2025Article
- Use of Subtherapeutic Tylvalosin AgainstTransboundary and emerging diseases · 2025Article
- Phenotypic and Genotypic Analysis of Antimicrobial Resistance inPathogens (Basel, Switzerland) · 2024Article
- Synergism between molecules derived from Garcinia brasiliensis and antimicrobial drugs on field isolates of Mycoplasma hyopneumoniae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Towards a Standardized Antimicrobial Susceptibility Testing Method forMicroorganisms · 2023Article
- A genetic and virulence characterization of Brazilian strains ofFrontiers in microbiology · 2023Article
- Oral Immunization with AttenuatedMicrobiology spectrum · 2022Article
- Review
- Development of a Multi-Epitope Vaccine forInternational journal of molecular sciences · 2022Article
- Characteristics ofFrontiers in veterinary science · 2022Article
- Detection ofCase reports in veterinary medicine · 2022Article
- Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera.BMC veterinary research · 2021Article
- Attenuation ofFrontiers in veterinary science · 2021Article
- Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions.Infection and immunity · 2020Article
- Antimicrobial susceptibility and genetic profile of Mycoplasma hyopneumoniae isolates from Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Genomic Variability and Post-translational Protein Processing Enhance the Immune Evasion ofFrontiers in immunology · 2020Review
- Development of an indirect ELISA for detecting humoral immunodominant proteins of Mycoplasma hyopneumoniae which can discriminate between inactivated bacterin-induced hyperimmune sera and convalescent sera.BMC veterinary research · 2019Article
- Mycoplasma hyopneumoniae evades phagocytic uptake by porcine alveolar macrophages in vitro.Veterinary research · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
The fastidious porcine respiratory pathogen Mycoplasma hyopneumoniae has proven difficult to culture since it was first isolated in 1965. A reliable solid medium has been particularly challenging. Moreover, clinical and pathological samples often contain the fast-growing M. hyorhinis which contaminates and overgrows M. hyopneumoniae in primary culture. The aim of this study was to optimise the culture medium for recovery of M. hyopneumoniae and to devise a medium for selection of M. hyopneumoniae from clinical samples also containing M. hyorhinis. The solid medium devised by Niels Friis was improved by use of Purified agar and incorporation of DEAE-dextran. Addition of glucose or neutralization of acidity in liquid medium with NaOH did not improve the final yield of viable organisms or alter the timing of peak viability. Analysis of the relative susceptibility of M. hyopneumoniae and M. hyorhinis strains to four antimicrobials showed that M. hyopneumoniae is less susceptible than M. hyorhinis to kanamycin. This was consistent in all UK and Danish strains tested. A concentration of 2μg/ml of kanamycin selectively inhibited the growth of all M. hyorhinis tested, while M. hyopneumoniae was able to grow. This forms the basis of an effective selective culture medium for M. hyopneumoniae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.