ArticleJournal of industrial microbiology & biotechnology2017
Regulation of crucial enzymes and transcription factors on 2-phenylethanol biosynthesis via Ehrlich pathway in Saccharomyces cerevisiae.
Article in Journal of industrial microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Triparental synthetic yeast hybrids as a platform for higher 2-phenylethanol yields and stress resistance.World journal of microbiology & biotechnology · 2026Article
- Fermentation Process Optimization for High 2-Phenylethanol Aroma Whisky.International journal of molecular sciences · 2026Article
- Voices of Eukaryotic Microbes: Chemical Communication Via Quorum Sensing.Microbial ecology · 2026Review
- EngineeringFrontiers in microbiology · 2026Review
- Endogenous pathway dysregulation in Kluyveromyces marxianus under nitrogen limitation drives 2-phenylethyl acetate and 2-phenylethanol overproduction.Scientific reports · 2025Article
- Possible regulatory network and associated pathways governing the expression of ADH2 in Saccharomyces cerevisiae.Current genetics · 2025Review
- Screening of High-Yield 2-Phenylethanol Producing Strain from Wild-TypeFoods (Basel, Switzerland) · 2025Article
- Transcriptomic Identification of Core Regulatory Genes for Higher Alcohol Production inFoods (Basel, Switzerland) · 2025Article
- Yeast-secreted compounds with antifungal activity-screening, genetic parts, biosynthetic pathways, and regulation.FEMS yeast research · 2025Review
- Biotechnological 2-Phenylethanol Production: Recent Developments.Molecules (Basel, Switzerland) · 2024Review
- Metabolic and tolerance engineering of Komagataella phaffii for 2-phenylethanol production through genome-wide scanning.Biotechnology for biofuels and bioproducts · 2024Article
- Transcriptomic and metabolomic correlation analysis: effect of initial SOFrontiers in microbiology · 2024Article
- Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway ofSynthetic and systems biotechnology · 2023Article
- Bioprinting microporous functional living materials from protein-based core-shell microgels.Nature communications · 2023Article
- Intestinal bacteria-a powerful weapon for fungal infections treatment.Frontiers in cellular and infection microbiology · 2023Review
- Quorum sensing: cell-to-cell communication inFrontiers in microbiology · 2023Review
- Effects of tryptophan and phenylalanine on tryptophol production in Saccharomyces cerevisiae revealed by transcriptomic and metabolomic analyses.Journal of microbiology (Seoul, Korea) · 2022Article
- Effect of Chilled Storage on Antioxidant Capacities and Volatile Flavors of Synbiotic Yogurt Made with Probiotic YeastJournal of fungi (Basel, Switzerland) · 2022Article
- Bioproduction of 2-Phenylethanol through Yeast Fermentation on Synthetic Media and on Agro-Industrial Waste and By-Products: A Review.Foods (Basel, Switzerland) · 2022Review
- Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors.Frontiers in microbiology · 2022Article
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Authors and funding
4 authors.
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Abstract
2-Phenylethanol (2-PE) is widely used in food, perfume and pharmaceutical industry, but lower production in microbes and less known regulatory mechanisms of 2-PE make further study necessary. In this study, crucial genes like ARO8 and ARO10 of Ehrlich pathway for 2-PE synthesis and key transcription factor ARO80 in Saccharomyces cerevisiae were re-regulated using constitutive promoter; in the meantime, the effect of nitrogen source in synthetic complete (SC) medium with L-phenylalanine (L-Phe) on Aro8/Aro9 and Aro10 was investigated. The results showed that aromatic aminotransferase activities of ARO8 over-expressing strains were seriously inhibited by ammonia sulfate in SC + Phe medium. Flask fermentation test demonstrated that over-expressing ARO8 or ARO10 led to about 42 % increase in 2-PE production when compared with the control strain. Furthermore, influence of transcription factors Cat8 and Mig1 on 2-PE biosynthesis was explored. CAT8 over-expression or MIG1 deletion increased in the transcription of ARO9 and ARO10. 2-PE production of CAT8 over-expressing strain was 62 % higher than that of control strain. Deletion of MIG1 also led to 2-PE biosynthesis enhancement. The strain of CAT8 over-expression and MIG1 deletion was most effective in regulating expression of ARO9 and ARO10. Analysis of mRNA levels and enzyme activities indicates that transaminase in Ehrlich pathway is the crucial target of Nitrogen Catabolize Repression (NCR). Among the engineering strains, the higher 3.73 g/L 2-PE production in CAT8 over-expressing strain without in situ product recovery suggests that the robust strain has potentiality for commercial exploitation.
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