ArticleMicrobial cell factories2016
A novel methanol-free Pichia pastoris system for recombinant protein expression.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Harnessing the PpHog1 Regulatory Network to Engineer a UPR-Adaptive Komagataella phaffii Chassis for High-Copy Secretory Protein Production.Microbial biotechnology · 2026Article
- Simultaneous GCN4 co-expression and promoter optimization enhance glucose oxidase production inIranian journal of microbiology · 2026Article
- Dual transcriptional and post-transcriptional regulation by the bHLH proteins Rtg1 and RtgX in Komagataella phaffii.Nucleic acids research · 2026Article
- Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.Biotechnology and bioengineering · 2025Article
- Design and construction of light-regulated gene transcription and protein translation systems in yeast P. Pastoris.Journal of advanced research · 2025Article
- Methanol-Free Protein Expression in Komagataella phaffii With Magnetic or Non-Magnetic Heating.Microbial biotechnology · 2025Article
- Effect of Fold-Promoting Mutation and Signal Peptide Screening on Recombinant Glucan 1,4-Alpha-maltohydrolase Secretion in Pichia pastoris.Applied biochemistry and biotechnology · 2025Article
- A self-inducible heterologous protein expression system in3 Biotech · 2024Article
- Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis.Microorganisms · 2024Review
- Unlocking Nature's Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter.Microbial cell factories · 2024Article
- Identification of a novel growth-associated promoter for biphasic expression of heterogenous proteins inApplied and environmental microbiology · 2024Article
- Use of an on/off tetracycline riboswitch to control protein production in Komagataella phaffii.AMB Express · 2023Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- High yield expression and purification of3 Biotech · 2023Article
- An inducible Komagataella phaffii system for protein expression using sorbitol dehydrogenase promoter.Biotechnology letters · 2023Article
- Enabling growth-decoupledFrontiers in bioengineering and biotechnology · 2023Article
- A programmable high-expression yeast platform responsive to user-defined signals.Science advances · 2022Article
- Current advances ofFrontiers in microbiology · 2022Review
- Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects.The protein journal · 2021Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAs one of the most popular expression systems, recombinant protein expression in Pichia pastoris relies on the AOX1 promoter (P
resultsWe identified two kinase mutants: Δgut1 and Δdak, both of which showed strong alcohol oxidase activity under non-methanol carbon sources. Based on these two kinases, we constructed two methanol-free expression systems: Δgut1-HpGCY1-glycerol (P
conclusionsWe successfully constructed a novel methanol-free expression system Δdak-DHA. This modified expression platform preserved the favorable regulatable nature of P
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.