Evidence map›Paper›PMID 27768949›Full record

ReviewCurrent opinion in chemical biology2016

Biofuel metabolic engineering with biosensors.

Stacy-Anne Morgan, Dana C Nadler, Rayka Yokoo, David F Savage

Abstract readReview
In one paragraph

Review in Current opinion in chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Congo Red Fluorescence for RapidApplied and environmental microbiology · 2019
    Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Stacy-Anne MorganDepartment of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA.
Dana C NadlerDepartment of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA.
Rayka YokooDepartment of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA.
David F SavageDepartment of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA; Energy Biosciences Institute, University of California, Berkeley, CA 94720, USA. Electronic address: dsavage@berkeley.edu.

Funding

Fluorescent biosensors for metabolite imaging in live cellsDP2EB018658 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI SAVAGE, DAVID FRANK · 2013 to 2013
$2.2M
NIBIB NIH HHS DP2 EB018658
6 · The paper itself

Abstract

Metabolic engineering offers the potential to renewably produce important classes of chemicals, particularly biofuels, at an industrial scale. DNA synthesis and editing techniques can generate large pathway libraries, yet identifying the best variants is slow and cumbersome. Traditionally, analytical methods like chromatography and mass spectrometry have been used to evaluate pathway variants, but such techniques cannot be performed with high throughput. Biosensors - genetically encoded components that actuate a cellular output in response to a change in metabolite concentration - are therefore a promising tool for rapid and high-throughput evaluation of candidate pathway variants. Applying biosensors can also dynamically tune pathways in response to metabolic changes, improving balance and productivity. Here, we describe the major classes of biosensors and briefly highlight recent progress in applying them to biofuel-related metabolic pathway engineering.

Indexed as

BiofuelsBiosensing TechniquesFluorescence Resonance Energy TransferMetabolic EngineeringRNABiofuelsRNA

Identifiers

PMID27768949
PMCPMC5161612

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.