Evidence map›Paper›PMID 27768827›Full record

ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2017

Cell size assays for mass cytometry.

Alan D Stern, Adeeb H Rahman, Marc R Birtwistle

Open access · greenAbstract read
In one paragraph

Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 67 citations in OpenAlex.

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  9. CyTOFFrontiers in immunology · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Alan D SternDepartment of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, New York, 10029.
Adeeb H RahmanDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, 10029.
Marc R BirtwistleDepartment of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, New York, 10029.
Icahn School of Medicine at Mount Sinai · US

Funding

Project 3 - Ex vivo immune profiling of dengue viruses and vaccinesU19AI118610 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KASARSKIS, ANDREW · 2015 to 2021
$46.6M
System Biology Center in New YorkP50GM071558 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI ZAPH, SUSANA NEVES · 2007 to 2017
$22.8M
Drug Combination Signatures for Prediction and Mitigation of ToxicityU54HG008098 · NHGRI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BIRTWISTLE, MARC R., SOBIE, ERIC A · 2014 to 2019
$11.8M
Teaching biomedical and pharmacological trainees to produce FAIR data for AI & ML applicationsT32GM062754 · NIGMS · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI SCHLESSINGER, AVNER · 2001 to 2023
$6.2M
Novel tools to maximize profiling of tissue and antigen specific immune dysregulation in allergy and inflammatory bowel disesaseU24AI118644 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BERIN, CECILIA, MERAD, MIRIAM · 2015 to 2020
$2.4M
Predicting the Heterogeneity of Cell-Fate DecisionsR01GM104184 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BIRTWISTLE, MARC R. · 2014 to 2018
$1.8M
Multiplexed, Quantitative Fluorescence Imaging in Tumor SectionsR21CA196418 · NCI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BIRTWISTLE, MARC R. · 2015 to 2017
$936k
NCI NIH HHS R21 CA196418NHGRI NIH HHS U54 HG008098NIAID NIH HHS U19 AI118610NIAID NIH HHS U24 AI118644NIGMS NIH HHS P50 GM071558NIGMS NIH HHS R01 GM104184NIGMS NIH HHS T32 GM062754
6 · The paper itself

Abstract

Mass cytometry offers the advantage of allowing the simultaneous measurement of a greater number parameters than conventional flow cytometry. However, to date, mass cytometry has lacked a reliable alternative to the light scatter properties that are commonly used as a cell size metric in flow cytometry (forward scatter intensity-FSC). Here, we report the development of two plasma membrane staining assays to evaluate mammalian cell size in mass cytometry experiments. One is based on wheat germ agglutinin (WGA) staining and the other on Osmium tetroxide (OsO

Indexed as

Cell SizeAnimalsCell MembraneFlow CytometryHumansOsmium TetroxideWheat Germ AgglutininsOsmium TetroxideWheat Germ Agglutininscell sizeCyTOFflow cytometryHEK293mass cytometryU87WGAwhole blood

Identifiers

PMID27768827
PMCPMC5250533
OpenAlexW2538538514

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.