ArticleJournal of visualized experiments : JoVE2016
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Structural and Functional Analysis of the Lectin-like Protein Llp1 Secreted byJournal of fungi (Basel, Switzerland) · 2025Article
- Structural and functional analysis of the cerato-platanin-like protein Cpl1 suggests diverging functions in smut fungi.Molecular plant pathology · 2023Article
- Engineering and Implementation of Synthetic Molecular Tools in the Basidiomycete FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Biogenesis of telomerase RNA from a protein-coding mRNA precursor.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Effector-mediated relocalization of a maize lipoxygenase protein triggers susceptibility to Ustilago maydis.The Plant cell · 2022Article
- Tetracycline-controlled (TetON) gene expression system for the smut fungusFrontiers in fungal biology · 2022Article
- A mixed-linkage (1,3;1,4)-β-D-glucan specific hydrolase mediates dark-triggered degradation of this plant cell wall polysaccharide.Plant physiology · 2021Article
- Controlling Unconventional Secretion for Production of Heterologous Proteins inJournal of fungi (Basel, Switzerland) · 2021Article
- Genetic Manipulation of the Brassicaceae Smut FungusJournal of fungi (Basel, Switzerland) · 2021Article
- A Novel Potent Carrier for Unconventional Protein Export inFrontiers in cell and developmental biology · 2021Article
- A Novel Factor Essential for Unconventional Secretion of Chitinase Cts1.Frontiers in microbiology · 2020Article
- New Insights of Ustilago maydis as Yeast Model for Genetic and Biotechnological Research: A Review.Current microbiology · 2019Review
- Marker-free genome editing in Ustilago trichophora with the CRISPR-Cas9 technology.RNA biology · 2019Article
- Single and Multiplexed Gene Editing inBio-protocol · 2018Article
- Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene deletion plays an important role in the analysis of gene function. One of the most efficient methods to disrupt genes in a targeted manner is the replacement of the entire gene with a selectable marker via homologous recombination. During homologous recombination, exchange of DNA takes place between sequences with high similarity. Therefore, linear genomic sequences flanking a target gene can be used to specifically direct a selectable marker to the desired integration site. Blunt ends of the deletion construct activate the cell's DNA repair systems and thereby promote integration of the construct either via homologous recombination or by non-homologous-end-joining. In organisms with efficient homologous recombination, the rate of successful gene deletion can reach more than 50% making this strategy a valuable gene disruption system. The smut fungus Ustilago maydis is a eukaryotic model microorganism showing such efficient homologous recombination. Out of its about 6,900 genes, many have been functionally characterized with the help of deletion mutants, and repeated failure of gene replacement attempts points at essential function of the gene. Subsequent characterization of the gene function by tagging with fluorescent markers or mutations of predicted domains also relies on DNA exchange via homologous recombination. Here, we present the U. maydis strain generation strategy in detail using the simplest example, the gene deletion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.