ArticleJournal of visualized experiments : JoVE2016
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Molecular mechanisms and applications of antimicrobial secondary metabolites ofApplied and environmental microbiology · 2026Review
- Differential coping strategies exerted by biofilm and planktonic cells ofMicrobiology spectrum · 2026Article
- Assessment of antibiofilm and quorum quenching potencies of environmental bacteria in controlling biofilm of food spoilage bacteria.BMC research notes · 2025Article
- The Utilization ofMicroorganisms · 2024Article
- Ice nucleation active bacteria metabolites as antibiofilm agent to control Aeromonas hydrophila and Streptococcus agalactiae infections in Aquaculture.BMC research notes · 2024Article
- Phyllosphere bacteria with antiquorum sensing and antibiofilm activities against fish pathogenic bacteria.BMC research notes · 2024Article
- Article
- Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation ineLife · 2022Article
- The roles of intracellular and extracellular calcium iniScience · 2022Article
- Integration of Transcriptome and Metabolome Reveals the Genes and Metabolites Involved inInternational journal of molecular sciences · 2021Article
- d-Amino Acids and Lactic Acid Bacteria.Microorganisms · 2019Review
- New Insights Into the Mechanisms and Biological Roles of D-Amino Acids in Complex Eco-Systems.Frontiers in microbiology · 2018Review
- Eradication ofFrontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work assesses different methodologies to study the impact of small molecule biofilm inhibitors, such as D-amino acids, on the development and resilience of Bacillus subtilis biofilms. First, methods are presented that select for small molecule inhibitors with biofilm-specific targets in order to separate the effect of the small molecule inhibitors on planktonic growth from their effect on biofilm formation. Next, we focus on how inoculation conditions affect the sensitivity of multicellular, floating B. subtilis cultures to small molecule inhibitors. The results suggest that discrepancies in the reported effects of such inhibitors such as D-amino acids are due to inconsistent pre-culture conditions. Furthermore, a recently developed protocol is described for evaluating the contribution of small molecule treatments towards biofilm resistance to antibacterial substances. Lastly, scanning electron microscopy (SEM) techniques are presented to analyze the three-dimensional spatial arrangement of cells and their surrounding extracellular matrix in a B. subtilis biofilm. SEM facilitates insight into the three-dimensional biofilm architecture and the matrix texture. A combination of the methods described here can greatly assist the study of biofilm development in the presence and absence of biofilm inhibitors, and shed light on the mechanism of action of these inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.