Evidence map›Paper›PMID 27768050›Full record

ArticleJournal of visualized experiments : JoVE2016

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina.

Hung-Ya Tu, Chih-Chun Hsu, Yu-Jiun Chen, Ching-Kang Chen

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hung-Ya TuDepartment of Ophthalmology, Baylor College of Medicine.
Chih-Chun HsuDepartment of Ophthalmology, Baylor College of Medicine; Department of Neuroscience, Baylor College of Medicine.
Yu-Jiun ChenDepartment of Ophthalmology, Baylor College of Medicine.
Ching-Kang ChenDepartment of Ophthalmology, Baylor College of Medicine; Department of Neuroscience, Baylor College of Medicine; Department of Biochemistry and Molecular Biology, Baylor College of Medicine; Ching-Kang.Chen@bcm.edu.

Funding

VISION RESEARCH CENTERP30EY002520 · NEI · BAYLOR COLLEGE OF MEDICINE · PI Samuel M Wu · 1985 to 2026
$14.8M
RECOVERY OF PHOTOTRANSDUCTIONR01EY013811 · NEI · VIRGINIA COMMONWEALTH UNIVERSITY · PI CHEN, CHING-KANG JASON · 2002 to 2017
$5.2M
Mechanisms, functions and utility of RGC oscillation in retinal deafferentation mouse modelsR01EY026930 · NEI · BAYLOR COLLEGE OF MEDICINE · PI CHEN, CHING-KANG JASON, WU, SAMUEL M · 2017 to 2019
$1.2M
The roles of Gbeta5 and R7 RGS protein in visionR01EY022228 · NEI · VIRGINIA COMMONWEALTH UNIVERSITY · PI CHEN, CHING-KANG JASON · 2012 to 2014
$1.1M
Recovery of PhototransductionR56EY013811 · NEI · VIRGINIA COMMONWEALTH UNIVERSITY · PI CHEN, CHING-KANG JASON · 2007 to 2007
$373k
NEI NIH HHS P30 EY002520NEI NIH HHS R01 EY013811NEI NIH HHS R01 EY022228NEI NIH HHS R01 EY026930NEI NIH HHS R56 EY013811
6 · The paper itself

Abstract

The mammalian retina is a layered tissue composed of multiple neuronal types. To understand how visual signals are processed within its intricate synaptic network, electrophysiological recordings are frequently used to study connections among individual neurons. We have optimized a flat-mount preparation for patch clamp recording of genetically marked neurons in both GCL (ganglion cell layer) and INL (inner nuclear layer) of mouse retinas. Recording INL neurons in flat-mounts is favored over slices because both vertical and lateral connections are preserved in the former configuration, allowing retinal circuits with large lateral components to be studied. We have used this procedure to compare responses of mirror-partnered neurons in retinas such as the cholinergic starburst amacrine cells (SACs).

Indexed as

Amacrine CellsPatch-Clamp TechniquesRetinaAnimalsCell Culture TechniquesMiceNeuronsRetinal Ganglion Cells

Identifiers

PMID27768050
PMCPMC5092195

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.