Evidence map›Paper›PMID 27767250›Full record

ArticleAngewandte Chemie (International ed. in English)2016

Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super-Resolution RESOLFT Microscopy.

Benoît Roubinet, Mariano L Bossi, Philipp Alt, Marcel Leutenegger, Heydar Shojaei, Sebastian Schnorrenberg, Shamil Nizamov, Masahiro Irie, Vladimir N Belov, Stefan W Hell

Abstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Turn-on type fluorescent photochromism of a diarylmaleimide-S,S,S',S'-tetraoxide.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025
    Article
  6. Article
  7. Article
  8. Review
  9. Near-infrared two-photon absorption and excited state dynamics of a fluorescent diarylethene derivative.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024
    Article
  10. Article
  11. Theranostic Fluorescent Probes.Chemical reviews · 2024
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Molecular photoswitches in aqueous environments.Chemical Society reviews · 2021
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Benoît RoubinetAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Mariano L BossiAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Philipp AltAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Marcel LeuteneggerAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Heydar ShojaeiAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Sebastian SchnorrenbergAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Shamil NizamovAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.
Masahiro IrieResearch Center for Smart Molecules, Department of Chemistry, Rikkyo University, Nishi-Ikebukuro 3-34-1, Toshimaku, Tokyo, Japan.
Vladimir N BelovAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.ORCID 0000-0002-7741-4653
Stefan W HellAbteilung Nanobiophotonik, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077, Göttingen, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.

Indexed as

diarylethenesfluorescenceoptical microscopyphotochromic dyeswater solubility

Identifiers

PMID27767250
PMCPMC5132007

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.