ArticleScientific reports2016
Bactrocera dorsalis male sterilization by targeted RNA interference of spermatogenesis: empowering sterile insect technique programs.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Article
- Reduction in Gonad Development and Sperm Motility in Male Brown PlanthopperInternational journal of molecular sciences · 2025Article
- Regulation of insect behavior by non-coding RNAs.Science China. Life sciences · 2024Review
- Diaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.GigaScience · 2024Article
- BmPMFBP1 regulates the development of eupyrene sperm in the silkworm, Bombyx mori.PLoS genetics · 2022Article
- Efficiency of RNA interference is improved by knockdown of dsRNA nucleases in tephritid fruit flies.Open biology · 2019Article
- Changes in Energy Metabolism Trigger Pupal Diapause Transition ofFrontiers in physiology · 2019Article
- RNA Interference-Mediated Knockdown of Male Fertility Genes in the Queensland Fruit FlyInsects · 2018Article
- Article
- BdorOBP2 plays an indispensable role in the perception of methyl eugenol by mature males of Bactrocera dorsalis (Hendel).Scientific reports · 2017Article
- Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference.Scientific reports · 2017Article
- Implementing the sterile insect technique with RNA interference - a review.Entomologia experimentalis et applicata · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) is a genetic technique which has novel application for sustainable pest control. The Sterile Insect Technique (SIT) uses releases of mass-produced, sterile male insects to out-compete wild males for mates to reduce pest populations. RNAi sterilization of SIT males would have several advantages over radiation sterilization, but to achieve this appropriate target genes must first be identified and then targeted with interference technology. With this goal, eight spermatogenesis related candidate genes were cloned and tested for potential activity in Bactrocera dorsalis. The knockdown of candidate genes by oral delivery of dsRNAs did not influence the mating of male flies, but significantly affected the daily average number of eggs laid by females, and reduced egg hatching rate by 16-60%. RNAi negatively affected spermatozoa quantitatively and qualitatively. Following the mating of lola-/topi-/rac-/rho-/upd-/magu-silenced males, we recorded a significant decrease in number and length of spermatozoa in female spermatheca compared to gfp-silenced control group. In a greenhouse trial, the number of damaged oranges and B. dorsalis larvae were significantly reduced in a dsrho-treated group compared with the dsgfp group. This study provides strong evidence for the use RNAi in pest management, especially for the improvement of SIT against B. dorsalis and other species.
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