Evidence map›Paper›PMID 27766489›Full record

ArticleClinical oral investigations2017

No evidence for the growth-stimulating effect of monomers on cariogenic Streptococci.

Ivana Nedeljkovic, Kumiko Yoshihara, Jan De Munck, Wim Teughels, Bart Van Meerbeek, Kirsten L Van Landuyt

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Ivana NedeljkovicBIOMAT, Department of Oral Health Sciences, University of Leuven and Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium.
Kumiko YoshiharaCenter for Innovative Clinical Medicine, Okayama University Hospital, 2-5-1 Shikata-cho, Okayama, Kita-ku, 700-8558,, Japan.
Jan De MunckBIOMAT, Department of Oral Health Sciences, University of Leuven and Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium.
Wim TeughelsOral Microbiology, Department of Oral Health Sciences, University of Leuven and Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium.
Bart Van MeerbeekBIOMAT, Department of Oral Health Sciences, University of Leuven and Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium.
Kirsten L Van LanduytBIOMAT, Department of Oral Health Sciences, University of Leuven and Dentistry University Hospitals Leuven, Kapucijnenvoer 7, 3000, Leuven, Belgium. kirsten.vanlanduyt@med.kuleuven.be.
KU Leuven · BEOkayama University Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn spite of contradicting results, the high susceptibility of composites for secondary caries is still often associated with the bacterial growth-stimulating effect of released methacrylate monomers. However, most studies that showed this effect were performed with techniques having inherent limitations (spectrophotometry).

objectivesTherefore, our objective was to determine the effect of four methacrylate monomers (2-Hydroxyethyl methacrylate (HEMA), triethylene glycol dimethacrylate (TEGDMA), ethylene glycol dimethacrylate (EGDMA), diethylene glycol dimethacrylate (DEGDMA)) on the growth of two caries-associated bacteria, Streptococcus mutans and sobrinus, and one non-cariogenic species, Streptococcus sanguinis, using TaqMan quantitative polymerase chain reaction (qPCR) to quantify bacterial DNA. MATERIALS AND

methodsCultures were exposed to monomer solutions selected after spectrophotometric growth measurements. At baseline and predetermined time intervals, bacterial DNA was extracted and quantified with TaqMan qPCR. Biofilms grown in the presence of monomers were analyzed with scanning electron microscopy (SEM).

resultsSpectrophotometry indeed showed increased growth rates of all three strains with 5 mM TEGDMA, EGDMA, and DEGDMA and increased total biomass of S. sanguinis with 5 mM TEGDMA. However, qPCR failed to show any growth-stimulating effect of these monomers on S. mutans and S. sobrinus. In contrast, some monomers exhibited a growth-inhibiting effect on S. sanguinis. SEM revealed extracellular matter in S. sobrinus and S. sanguinis biofilms, which might be attributed to polymer formation.

conclusionsTechniques which quantify bacterial DNA are more appropriate to evaluate bacterial growth in the presence of monomers than spectrophotometry. CLINICAL RELEVANCE: Even though methacrylate monomers did not affect the growth of cariogenic species, growth inhibition of S. sanguinis, a non-cariogenic antagonistic species, may lead to ecological shifts towards higher cariogenicity.

Indexed as

BiofilmsComposite ResinsDental CariesDNA, BacterialMethacrylatesMethylmethacrylateMicroscopy, Electron, ScanningPolyethylene GlycolsPolymethacrylic AcidsReal-Time Polymerase Chain ReactionSpectrophotometryStreptococcus mutansStreptococcus sanguisStreptococcus sobrinusComposite ResinsDNA, Bacterialethylene dimethacrylatehydroxyethyl methacrylateMethacrylatesMethylmethacrylatePolyethylene GlycolsPolymethacrylic Acidstriethylene glycol dimethacrylateBacterial growthDental compositeMethacrylate monomersOral streptococciqPCRRT PCRSpectrophotometryStreptococcus mutansStreptococcus sanguinisStreptococcus sobrinus

Identifiers

PMID27766489
OpenAlexW2536426716

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.