ArticleDyes and pigments : an international journal2016
Hydrophobic resorufamine derivatives: potent and selective red fluorescent probes of the endoplasmic reticulum of mammalian cells.
Article in Dyes and pigments : an international journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Designer Aromatic Cations for Photoinduced Protein Ligation, Imaging, and Intracellular Labeling at Extended Wavelengths.Journal of the American Chemical Society · 2026Article
- Exploiting Flavylium Merocyanine Dyes for Intrinsic, Multiplexed Labeling of the Endoplasmic Reticulum.Analytical chemistry · 2025Article
- Advances in Organic Fluorescent Probes for Intracellular ZnMolecules (Basel, Switzerland) · 2024Review
- Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging.Biosensors · 2023Review
- Organelle Interaction and Drug Discovery: Towards Correlative Nanoscopy and Molecular Dynamics Simulation.Frontiers in pharmacology · 2022Review
- An aggregation-induced emission platform for efficient Golgi apparatus and endoplasmic reticulum specific imaging.Chemical science · 2021Article
- Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes.World journal of gastroenterology · 2020Article
- Fluorescent detection of peroxynitrite during antibody-dependent cellular phagocytosis.Methods in enzymology · 2020Article
- Endoplasmic reticulum targeting fluorescent probes to image mobile ZnChemical science · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The endoplasmic reticulum (ER) of eukaryotic cells plays critical roles in the processing of secreted and transmembrane proteins. Defects in these functions are associated with a wide range of pathologies. To image this organelle, cells are often treated with fluorescent ER-Tracker dyes. Although these compounds are selective, existing red fluorescent probes of the ER are costly glibenclamide derivatives that inhibit ER-associated sulphonylurea receptors. To provide simpler and more cost-effective red fluorescent probes of the ER, we synthesized amino analogues of the fluorophore resorufin. By varying the polarity of linked substituents, we identified hexyl resorufamine (HRA) as a novel hydrophobic (cLogD (pH 7.4) = 3.8) red fluorescent (Ex. 565 nm; Em. 614 nm in ethanol) molecular probe. HRA is exceptionally bright in organic solvents (quantum yield = 0.70), it exclusively localizes to the ER of living HeLa cells as imaged by confocal microscopy, it is effective at concentrations as low as 100 nM, and it is non-toxic under these conditions. To examine its utility, we used HRA to facilitate visualization of small molecule-mediated release of a GFP-GPI fusion protein from the ER into the secretory pathway. HRA represents a potent, selective, and cost-effective probe for imaging and labeling the ER.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.