ArticleScientific reports2016
Different populations of Wnt-containing vesicles are individually released from polarized epithelial cells.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Membrane lipid composition and rapid endocytic retrieval modulate Wingless secretion.The EMBO journal · 2026Article
- Primary cilia-extracellular vesicle crosstalk in Alzheimer's disease: Emerging mechanisms and biomarker potential.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026Review
- Regioselective Synthesis of Ambipolar B-N Lewis Pair Functionalized Pyrenes: Structural Dynamics, Emission Tuning, and Applications in Live Cell Imaging and as Electrochemiluminescent Materials.Journal of the American Chemical Society · 2026Article
- Telocytes deliver essential Wnts directly to murine intestinal stem cells via synapse-like contacts.Developmental cell · 2025Article
- Wnt signaling - using the bloodstream to send a message.Cellular and molecular life sciences : CMLS · 2025Review
- Article
- Role of exosomes in gastrointestinal physiology and pathophysiology.Frontiers in immunology · 2025Review
- The biology of exosomes and exosomal non-coding RNAs in cardiovascular diseases.Frontiers in pharmacology · 2025Review
- Extracellular vesicle therapy in neurological disorders.Journal of biomedical science · 2024Review
- Cilia and Extracellular Vesicles in Brain Development and Disease.Biological psychiatry · 2024Review
- Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretion.Communications biology · 2024Article
- Two roads diverged in a cell: insights from differential exosome regulation in polarized cells.Frontiers in cell and developmental biology · 2024Review
- Polarized Desmosome and Hemidesmosome Shedding via Small Extracellular Vesicles is an Early Indicator of Outer Blood-Retina Barrier Dysfunction.Journal of extracellular biology · 2023Article
- Emerging connections between GPI-anchored proteins and their extracellular carriers in colorectal cancer.Extracellular vesicles and circulating nucleic acids · 2023Article
- Antiparallel dimer structure of CELSR cadherin in solution revealed by high-speed atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Extracellular Vesicles in Breast Cancer: From Biology and Function to Clinical Diagnosis and Therapeutic Management.International journal of molecular sciences · 2023Review
- Controlled release of canine MSC-derived extracellular vesicles by cationized gelatin hydrogels.Regenerative therapy · 2023Article
- Mesenchymal Stem Cells and Their Exocytotic Vesicles.International journal of molecular sciences · 2023Review
- Challenges for Studying and Isolating Extracellular Vesicles from Cell-Conditioned Media.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Scalable Production of Extracellular Vesicles and Its Therapeutic Values: A Review.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accumulating evidence suggests that exosomes are heterogeneous in molecular composition and physical properties. Here we examined whether epithelial cells secrete a heterogeneous population of exosomes, and if that is the case, whether epithelial cell polarity affects release of different populations of exosomes, especially that of those carrying Wnt. Sucrose-density ultracentrifugation and molecular marker analysis revealed that different populations of exosomes or exosome-like vesicles were released from MDCK cells depending on the cell polarity. Wnt3a associated with these vesicles were detectable in culture media collected from both apical and basolateral sides of the cells. Basolaterally secreted Wnt3a were co-fractionated with a typical exosomal protein TSG101 in fractions having typical exosome densities. In contrast, most of apically secreted Wnt3a, as well as Wnt11, were co-fractionated with CD63 and Hsp70, which are also common to the most exosomes, but recovered in higher density fractions. Wnt3a exhibiting similar floatation behavior to the apically secreted ones were also detectable in the culture media of Wnt3a-expressing L and HEK293 cells. The lipidation of Wnt3a was required for its basolateral secretion in exosomes but was dispensable for the apical one. Thus, epithelial cells release Wnt via distinct populations of vesicles differing in secretion polarity and lipidation dependency.
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Registered trials
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