ArticleScientific reports2016
Integrating Candida albicans metabolism with biofilm heterogeneity by transcriptome mapping.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- In Vitro Exposure to Vaped Tetrahydrocannabinol IncreasesMicroorganisms · 2025Article
- Fungal biofilms in human health and disease.Nature reviews. Microbiology · 2025Review
- Metabolic reprogramming during Candida albicans planktonic-biofilm transition is modulated by the transcription factors Zcf15 and Zcf26.PLoS biology · 2024Article
- Secreted Aspartic Proteinases: Key Factors inInternational journal of molecular sciences · 2024Review
- Decoding the role of oxidative stress resistance and alternative carbon substrate assimilation in the mature biofilm growth mode of Candida glabrata.BMC microbiology · 2024Article
- Review
- An integrated transcriptomic and metabolomic approach to investigate the heterogeneousBiofilm · 2023Article
- The Interplay betweenMicroorganisms · 2023Review
- Impaired amino acid uptake leads to global metabolic imbalance of Candida albicans biofilms.NPJ biofilms and microbiomes · 2022Article
- Assessment of Biofilm Formation byJournal of fungi (Basel, Switzerland) · 2022Article
- Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence.Metallomics : integrated biometal science · 2022Article
- Comparative Transcriptomics Reveal Possible Mechanisms of Amphotericin B Resistance in Candida auris.Antimicrobial agents and chemotherapy · 2022Article
- Article
- Review
- Article
- The Transcription Factor Stp2 Is Important forFrontiers in microbiology · 2020Article
- Phenotypic characteristics and transcriptome profile of Cryptococcus gattii biofilm.Scientific reports · 2019Article
- The Significance of Lipids to Biofilm Formation inJournal of fungi (Basel, Switzerland) · 2018Review
- Transcriptome Assembly and Profiling ofmSphere · 2018Article
- Gaining Insights from Candida Biofilm Heterogeneity: One Size Does Not Fit All.Journal of fungi (Basel, Switzerland) · 2018Review
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
Abstract
Candida albicans biofilm formation is an important virulence factor in the pathogenesis of disease, a characteristic which has been shown to be heterogeneous in clinical isolates. Using an unbiased computational approach we investigated the central metabolic pathways driving biofilm heterogeneity. Transcripts from high (HBF) and low (LBF) biofilm forming isolates were analysed by RNA sequencing, with 6312 genes identified to be expressed in these two phenotypes. With a dedicated computational approach we identified and validated a significantly differentially expressed subnetwork of genes associated with these biofilm phenotypes. Our analysis revealed amino acid metabolism, such as arginine, proline, aspartate and glutamate metabolism, were predominantly upregulated in the HBF phenotype. On the contrary, purine, starch and sucrose metabolism was generally upregulated in the LBF phenotype. The aspartate aminotransferase gene AAT1 was found to be a common member of these amino acid pathways and significantly upregulated in the HBF phenotype. Pharmacological inhibition of AAT1 enzyme activity significantly reduced biofilm formation in a dose-dependent manner. Collectively, these findings provide evidence that biofilm phenotype is associated with differential regulation of metabolic pathways. Understanding and targeting such pathways, such as amino acid metabolism, is potentially useful for developing diagnostics and new antifungals to treat biofilm-based infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.