ArticleParasites & vectors2016
Anaplasma marginale and A. phagocytophilum in cattle in Tunisia.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Effects of cattle on vector-borne disease risk to humans: A systematic review.PLoS neglected tropical diseases · 2023Pooled it
- Occurrence of tick-borne haemoparasites in South African rodent species evidence ofInternational journal for parasitology. Parasites and wildlife · 2025Article
- What is the value of testing for tick-borne diseases in cattle in endemic areas? A case study of bovine anaplasmosis.PloS one · 2025Article
- CRISPR-Cas-Based Pen-Side Diagnostic Tests forMicroorganisms · 2024Article
- Molecular Detection ofVeterinary medicine international · 2024Article
- Dynamics of Infections in Cattle andPathogens (Basel, Switzerland) · 2023Article
- Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges.One health (Amsterdam, Netherlands) · 2023Review
- Review
- Unravelling the diversity ofCurrent research in microbial sciences · 2023Article
- Distribution of Tick-Borne Pathogens in Domestic Animals and Their Ticks in the Countries of the Mediterranean Basin between 2000 and 2021: A Systematic Review.Microorganisms · 2022Article
- First Molecular Evidence for the Presence ofPathogens (Basel, Switzerland) · 2022Article
- Article
- Article
- Development of a duplex PCR assay for detecting Theileria luwenshuni and Anaplasma phagocytophilum in sheep and goats.Experimental & applied acarology · 2021Article
- Molecular Survey ofVeterinary sciences · 2021Article
- Article
- Cross-sectional survey of cattle haemopathogens in Constantine, Northeast Algeria.Veterinary medicine and science · 2021Article
- Prevalence of antibodies toJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Serological and molecular surveys ofIranian journal of veterinary research · 2021Article
- Screening and Analysis ofFrontiers in veterinary science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTick-borne diseases caused by Anaplasma species put serious constraints on the health and production of domestic cattle in tropical and sub-tropical regions. After recovering from a primary infection, cattle typically become persistent carriers of pathogens and play a critical role in the epidemiology of the disease, acting as reservoirs of the Anaplasma spp.
methodsIn this study a duplex PCR assay was used for the simultaneous detection of Anaplasma marginale and Anaplasma phagocytophilum in cattle using two primer pairs targeting msp4 and msp2 genes, respectively. We used this method to analyze DNA preparations derived from 328 blood cattle samples that were collected from 80 farms distributed among Tunisia's four bioclimatic zones.
resultsThe prevalence of the A. marginale infection (24.7 %) was significantly higher and more widespread (in all bioclimatic areas) than that of A. phagocytophilum (0.6 %), which was found in a mixed infection with A. marginale.
conclusionsThe duplex PCR assay used proved to be a rapid, specific and inexpensive mean for the simultaneous detection of Anaplasma marginale and Anaplasma phagocytophilum in cattle blood. It allowed us to report the identification of A. phagocytophilum for the first time in cattle in Tunisia and confirm the presence of A. marginale in cattle from several geographical areas of the country. Further epidemiological studies undertaken using this assay will help improve the surveillance of the associated diseases in the regions where they are endemic.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.