Evidence map›Paper›PMID 27765038›Full record

ArticleRespiratory research2016

T cells suppress memory-dependent rapid mucous cell metaplasia in mouse airways.

Hitendra S Chand, Yohannes A Mebratu, Marena Montera, Yohannes Tesfaigzi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Respiratory research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Hitendra S ChandCOPD Program, Lovelace Respiratory Research Institute, Albuquerque, NM, 87108, USA. hchand@fiu.edu.
Yohannes A MebratuCOPD Program, Lovelace Respiratory Research Institute, Albuquerque, NM, 87108, USA.
Marena MonteraCOPD Program, Lovelace Respiratory Research Institute, Albuquerque, NM, 87108, USA.
Yohannes TesfaigziCOPD Program, Lovelace Respiratory Research Institute, Albuquerque, NM, 87108, USA.
Lovelace Respiratory Research Institute · USFlorida International University · US

Funding

Resolution of Epithelial Cell Hyperplasia in AsthmaR01HL068111 · NHLBI · LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE · PI TESFAIGZI, YOHANNES · 2003 to 2021
$10.9M
Resolution of Epithelial Cell HyperplasiaR01ES015482 · NIEHS · LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE · PI TESFAIGZI, YOHANNES · 2009 to 2013
$2.6M
Bik Promotes Cleavage of Viral Proteins to Enhance Influenza A Virus InfectionR21AI115291 · NIAID · LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE · PI MEBRATU, YOHANNES AFEWORK · 2015 to 2016
$600k
Targeting Hyperplastic Airway Cells Using a Small Molecule InhibitorR21AI117560 · NIAID · LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE · PI CHAND, HITENDRA SINGH · 2016 to 2017
$510k
Regulation of Mucous Cell Metaplasia in AsthmaR56HL068111 · NHLBI · LOVELACE BIOMEDICAL & ENVIRONMENTAL RESEARCH INSTITUTE · PI TESFAIGZI, YOHANNES · 2007 to 2007
$500k
NHLBI NIH HHS R01 HL068111NHLBI NIH HHS R56 HL068111NIAID NIH HHS R21 AI115291NIAID NIH HHS R21 AI117560NIEHS NIH HHS R01 ES015482
6 · The paper itself

Abstract

backgroundAirway epithelial cells (AECs) are crucial for mucosal and adaptive immunity but whether these cells respond in a memory-dependent manner is poorly studied. Previously, we have reported that LPS intratracheal instillation in rodents causes extensive neutrophilic inflammation and airway epithelial cell hyperplasia accompanied by mucous cell metaplasia (MCM). And the resolution process required a period of 40 d for the inflammation to subside and the lung epithelia to resemble the non-exposed condition. Therefore, the present study investigated the memory-dependent response of airway epithelial cells to a secondary LPS challenge after the initial inflammation was resolved.

methodsAirway epithelial and mucous cells were assessed in response to a secondary LPS challenge in F344/N rats, and in C57BL/6 wild-type (Foxn1

resultsLPS-pretreated F344/N rats responded with elevated numbers of AECs after saline challenge and with 3-4-fold increased MCM following the LPS challenge in LPS- compared with saline-pretreated rats. LPS-pretreated rats showed 5-fold higher number of AECs expressing TLR4 apically than saline-pretreated rats. Also, the expression of EGFR was increased in LPS-pretreated rats along with the number of AECs with active or nuclear pERK, and the levels were further increased upon LPS challenge. LPS-pretreated Foxn1

conclusionTogether, these data suggest that AECs preserve an 'innate memory' that drives a rapid mucous phenotype via spatiotemporal regulation of TLR4 and EGFR. Further, T cells may suppress the sustained elevated expression of TLR4 and EGFR and thereby the hyperactive epithelial response.

Indexed as

Immunity, MucosalImmunologic MemoryAnimalsDisease Models, AnimalEpithelial CellsErbB ReceptorsForkhead Transcription FactorsLipopolysaccharidesMaleMetaplasiaMice, Inbred C57BLMice, KnockoutMice, NudeMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PhosphorylationEGFR protein, mouseErbB ReceptorsForkhead Transcription FactorsLipopolysaccharidesMapk1 protein, mouseMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Tlr4 protein, mouseToll-Like Receptor 4Whn proteinEpidermal growth factor receptorEpithelial memoryLPSMucous cell metaplasiaT cellsToll-like receptor 4

Identifiers

PMID27765038
PMCPMC5073838
OpenAlexW2539105881

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.