ArticleAsian-Australasian journal of animal sciences2017
Myostatin gene knockout mediated by Cas9-D10A nickase in chicken DF1 cells without off-target effect.
Article in Asian-Australasian journal of animal sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Comprehensive transcriptomic analysis of myostatin-knockout pigs: insights into muscle growth and lipid metabolism.Transgenic research · 2025Article
- CRISPR/Cas9-based programmable genome editing in chickens: concepts, applications and regulatory issues.Frontiers in genome editing · 2025Review
- - Invited Review - Gene-editing techniques and their applications in livestock and beyond.Animal bioscience · 2023Article
- Article
- Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species.Frontiers in genetics · 2021Review
- Muscle differentiation induced by p53 signaling pathway-related genes in myostatin-knockout quail myoblasts.Molecular biology reports · 2020Article
- Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts.BMC molecular and cell biology · 2020Article
- CRISPR/Cas9 gene editing in a chicken model: current approaches and applications.Journal of applied genetics · 2020Review
- EffectiveInternational journal of molecular sciences · 2020Article
- CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities.Frontiers in genetics · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveBased on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells.
methodsChicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (
resultsThrough the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed.
conclusionThe knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.
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