Evidence map›Paper›PMID 27764552›Full record

ArticleGM crops & food2016

Molecular and functional characterization of cry1Ac transgenic pea lines.

Alemayehu Teressa Negawo, Linda Baranek, Hans-Jörg Jacobsen, Fathi Hassan

Abstract read
In one paragraph

Article in GM crops & food, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alemayehu Teressa Negawoa Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.
Linda Baraneka Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.
Hans-Jörg Jacobsena Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.
Fathi Hassana Institute for Plant Genetics (Section of Plant Biotechnology), Gottfried Wilhelm Leibniz University of Hannover , Hannover , Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transgenic pea lines transformed with the cry1Ac gene were characterized at molecular (PCR, RT-PCR, qRT-PCR and immunostrip assay) and functional levels (leaf paint and insect feeding bioassays). The results showed the presence, expression, inheritance and functionality of the introduced transgene at different progeny levels. Variation in the expression of the cry1Ac gene was observed among the different transgenic lines. In the insect bioassay studies using the larvae of Heliothis virescens, both larval survival and plant damage were highly affected on the different transgenic plants. Up to 100 % larval mortality was observed on the transgenic plants compared to 17.42 % on control plants. Most of the challenged transgenic plants showed very negligible to substantially reduced feeding damage indicating the insect resistance of the developed transgenic lines. Further analysis under field condition will be required to select promising lines for future uses.

Indexed as

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin ProteinsLarvaMothsPest Control, BiologicalPisum sativumPlant DiseasesPlants, Genetically ModifiedTransgenesBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus Thuringiensiscry1Ac proteinfeeding damageinsect pestslarval mortalityPeatransgenic resistance

Identifiers

PMID27764552
PMCPMC5161004

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.