ArticlePloS one2016
Comparative Analysis of Membrane Vesicles from Three Piscirickettsia salmonis Isolates Reveals Differences in Vesicle Characteristics.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Differential Transcriptomic Profile ofMicroorganisms · 2024Article
- [Cu(NNInternational journal of molecular sciences · 2024Article
- Outer Membrane Vesicles of Actinobacillus pleuropneumoniae Exert Immunomodulatory Effects on Porcine Alveolar Macrophages.Microbiology spectrum · 2022Article
- Collective behavior and virulence arsenal of the fish pathogenFrontiers in cellular and infection microbiology · 2022Article
- Prochlorococcus extracellular vesicles: molecular composition and adsorption to diverse microbes.Environmental microbiology · 2022Article
- Comparative Analysis of Salmon Cell Lines and Zebrafish Primary Cell Cultures Infection with the Fish PathogenMicroorganisms · 2021Article
- The Phagosome-Lysosome Fusion Is the Target of a PurifiedAntibiotics (Basel, Switzerland) · 2021Article
- Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages.Frontiers in microbiology · 2021Article
- Engineering a defined culture medium to grow Piscirickettsia salmonis for its use in vaccine formulations.Journal of industrial microbiology & biotechnology · 2020Article
- Isolation and Characterization of Serum Extracellular Vesicles (EVs) from Atlantic Salmon Infected with Piscirickettsia Salmonis.Proteomes · 2017Article
- The Proteome of Biologically Active Membrane Vesicles fromFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membrane vesicles (MVs) are spherical particles naturally released from the membrane of Gram-negative bacteria. Bacterial MV production is associated with a range of phenotypes including biofilm formation, horizontal gene transfer, toxin delivery, modulation of host immune responses and virulence. This study reports comparative profiling of MVs from bacterial strains isolated from three widely disperse geographical areas. Mass spectrometry identified 119, 159 and 142 proteins in MVs from three different strains of Piscirickettsia salmonis isolated from salmonids in Chile (LF-89), Norway (NVI 5692) and Canada (NVI 5892), respectively. MV comparison revealed several strain-specific differences related to higher virulence capability for LF-89 MVs, both in vivo and in vitro, and stronger similarities between the NVI 5692 and NVI 5892 MV proteome. The MVs were similar in size and appearance as analyzed by electron microscopy and dynamic light scattering. The MVs from all three strains were internalized by both commercial and primary immune cell cultures, which suggest a potential role of the MVs in the bacterium's utilization of leukocytes. When MVs were injected into an adult zebrafish infection model, an upregulation of several pro-inflammatory genes were observed in spleen and kidney, indicating a modulating effect on the immune system. The present study is the first comparative analysis of P. salmonis derived MVs, highlighting strain-specific vesicle characteristics. The results further illustrate that the MV proteome from one bacterial strain is not representative of all bacterial strains within one species.
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