ArticlePloS one2016
Whole-Genome Resequencing of a Cucumber Chromosome Segment Substitution Line and Its Recurrent Parent to Identify Candidate Genes Governing Powdery Mildew Resistance.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Genomics assisted breeding for mildew resistance in cucumber: from gene discovery to future innovations.Plant molecular biology · 2026Review
- Phytoalexin deficient 4 is associated with the lesion mimic trait in watermelon clalm mutant (Citrullus lanatus).BMC plant biology · 2025Article
- Development of cleaved amplified polymorphic sequence marker for powdery mildew resistance in Korean malting barley using QTL-seq.Frontiers in plant science · 2025Article
- Article
- The effect of environmental factors on the genetic differentiation of Cucurbita ficifolia populations based on whole-genome resequencing.BMC plant biology · 2023Article
- Research Advances in Genetic Mechanisms of Major Cucumber Diseases Resistance.Frontiers in plant science · 2022Review
- Transgressive resistance to Heterodera glycines in chromosome segment substitution lines derived from susceptible soybean parents.The plant genome · 2021Article
- QTL-seq analysis of powdery mildew resistance in a Korean cucumber inbred line.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Comparative analysis of powdery mildew resistant and susceptible cultivated cucumber (Cucumis sativus L.) varieties to reveal the metabolic responses to Sphaerotheca fuliginea infection.BMC plant biology · 2021Article
- Localization of quantitative trait loci for cucumber fruit shape by a population of chromosome segment substitution lines.Scientific reports · 2020Article
- Combined Linkage Mapping and BSA to Identify QTL and Candidate Genes for Plant Height and the Number of Nodes on the Main Stem in Soybean.International journal of molecular sciences · 2019Article
- Development of Three Sets of High-Throughput Genotyped Rice Chromosome Segment Substitution Lines and QTL Mapping for Eleven Traits.Rice (New York, N.Y.) · 2019Article
- Development and use of chromosome segment substitution lines as a genetic resource for crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Genetic mapping ofMolecular breeding : new strategies in plant improvement · 2018Article
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7 authors.
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Abstract
Cucumber is an economically important vegetable crop worldwide. Powdery mildew (PM) is one of the most severe diseases that can affect cucumber crops. There have been several research efforts to isolate PM resistance genes for breeding PM-resistant cucumber. In the present study, we used a chromosome segment substitution line, SSL508-28, which carried PM resistance genes from the donor parent, JIN5-508, through twelve generations of backcrossing with a PM-susceptible inbred line, D8. We performed whole-genome resequencing of SSL508-28 and D8 to identify single nucleotide polymorphisms (SNPs), and insertions and deletions (indels). When compared against the reference genome of the inbred cucumber line 9930, a total of 468,616 SNPs and 67,259 indels were identified in SSL508-28, and 537,352 SNPs and 91,698 indels were identified in D8. Of these, 3,014 non-synonymous SNPs and 226 frameshift indels in SSL508-28, and 3,104 non-synonymous SNPs and 251 frameshift indels in D8, were identified. Bioinformatics analysis of these variations revealed a total of 15,682 SNPs and 6,262 indels between SSL508-28 and D8, among which 120 non-synonymous SNPs and 30 frameshift indels in 94 genes were detected between SSL508-28 and D8. Finally, out of these 94 genes, five resistance genes with nucleotide-binding sites and leucine-rich repeat domains were selected for qRT-PCR analysis. This revealed an upregulation of two transcripts, Csa2M435460.1 and Csa5M579560.1, in SSL508-28. Furthermore, the results of qRT-PCR analysis of these two genes in ten PM resistant and ten PM susceptible cucumber lines showed that when exposed to PM, Csa2M435460.1 and Csa5M579560.1 exhibited a higher expression level of resistant lines than susceptible lines. This indicates that Csa2M435460.1 and Csa5M579560.1 are candidate genes for PM resistance in cucumber. In addition, the non-synonymous SNPs in Csa2M435460.1 and Csa5M579560.1, identified in SSL508-28 and D8, might be the key to high PM-resistance in SSL508-28.
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