ArticleNature protocols2016
Assembly of multienzyme complexes on DNA nanostructures.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 116 citations in OpenAlex.
- Construction of a COChemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Modular programming of interaction and geometric specificity enables assembly of complex DNA origami nanostructures.Nature communications · 2025Article
- Analyzing DNA Origami Nanostructure Assembly by Dynamic Light Scattering and Nanoparticle Tracking Analysis.Small methods · 2025Article
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- A method for site-specifically tethering the enzyme urease to DNA origami with sustained activity.PloS one · 2025Article
- A Robust and Efficient Method to Purify DNA-Scaffolded Nanostructures by Gravity-Driven Size Exclusion Chromatography.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Multivalent insulin receptor activation using insulin-DNA origami nanostructures.Nature nanotechnology · 2024Article
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- Single-Molecule FRET-Based Multiplexed Detection.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells.Biosensors · 2023Review
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- The wending rhombus: Self-assembling 3D DNA crystals.Biophysical journal · 2022Review
- Article
- Inactivation Kinetics of G-Quadruplex/Hemin Complex and Optimization for More Reliable Catalysis.ChemPlusChem · 2022Article
- The role of DNA nanostructures in the catalytic properties of an allosterically regulated protease.Science advances · 2022Article
- Single-Molecule FRET: A Tool to Characterize DNA Nanostructures.Frontiers in molecular biosciences · 2022Review
- Applications of DNA-Functionalized Proteins.International journal of molecular sciences · 2021Review
- Single-Molecule FRET-Based Dynamic DNA Sensor.ACS sensors · 2021Article
- DNA-Scaffolded Proximity Assembly and Confinement of Multienzyme Reactions.Topics in current chemistry (Cham) · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In nature, the catalytic efficiency of multienzyme complexes highly depends on their spatial organization. The positions and orientations of the composite enzymes are often precisely controlled to facilitate substrate transport between them. Self-assembled DNA nanostructures hold great promise for organizing biomolecules at the nanoscale. Here, we present detailed protocols for exploiting DNA nanostructures as assembly scaffolds that organize the spatial arrangements of multienzyme cascades with control over their relative distance, compartmentalization and substrate diffusion paths. The protocol describes the preparation and purification of DNA-conjugated enzymes and cofactors, along with the assembly of these prepared complexes on DNA nanostructures. The architecture of assembled enzyme complexes is then readily characterized using a broad selection of techniques from routine gel electrophoresis to advanced single-molecule imaging. We also describe methods of purifying these nano-assemblies and testing them with functional assays based on either bulk or single-molecule fluorescence measurements. The entire assembly and characterization of a multienzyme complex can be completed within 1-2 weeks.
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