ArticleScientific reports2016
Cryo-EM structure of the large subunit of the spinach chloroplast ribosome.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Mapping the architecture of protein complexes inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Defective Processing of Cytoplasmic and Chloroplast Ribosomal RNA in the Absence of Arabidopsis DXO1.Plant, cell & environment · 2025Article
- Diversification of Plastid Structure and Function in Land Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Chloroplast gene expression: Recent advances and perspectives.Plant communications · 2023Review
- Perturbation of protein homeostasis brings plastids at the crossroad between repair and dismantling.PLoS genetics · 2023Article
- Plastid ribosome protein L5 is essential for post-globular embryo development in Arabidopsis thaliana.Plant reproduction · 2022Article
- CryoDRGN: reconstruction of heterogeneous cryo-EM structures using neural networks.Nature methods · 2021Article
- LSTrAP-Crowd: prediction of novel components of bacterial ribosomes with crowd-sourced analysis of RNA sequencing data.BMC biology · 2020Article
- Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes.Plants (Basel, Switzerland) · 2020Article
- The Arabidopsis Protein CGL20 Is Required for Plastid 50S Ribosome Biogenesis.Plant physiology · 2020Article
- Ribosome-Associated Chloroplast SRP54 Enables Efficient Cotranslational Membrane Insertion of Key Photosynthetic Proteins.The Plant cell · 2019Article
- Citrus exocortis viroid causes ribosomal stress in tomato plants.Nucleic acids research · 2019Article
- Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA.Scientific reports · 2018Article
- CHLOROPLAST RIBOSOME ASSOCIATED Supports Translation under Stress and Interacts with the Ribosomal 30S Subunit.Plant physiology · 2018Article
- Real-space refinement in PHENIX for cryo-EM and crystallography.Acta crystallographica. Section D, Structural biology · 2018Article
- Chloroplast Translation: Structural and Functional Organization, Operational Control, and Regulation.The Plant cell · 2018Review
- Pausing of Chloroplast Ribosomes Is Induced by Multiple Features and Is Linked to the Assembly of Photosynthetic Complexes.Plant physiology · 2018Article
- Article
- Functional characterization of chloroplast-targeted RbgA GTPase in higher plants.Plant molecular biology · 2017Article
- Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.Nucleic acids research · 2017Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein synthesis in the chloroplast is mediated by the chloroplast ribosome (chloro-ribosome). Overall architecture of the chloro-ribosome is considerably similar to the Escherichia coli (E. coli) ribosome but certain differences are evident. The chloro-ribosome proteins are generally larger because of the presence of chloroplast-specific extensions in their N- and C-termini. The chloro-ribosome harbours six plastid-specific ribosomal proteins (PSRPs); four in the small subunit and two in the large subunit. Deletions and insertions occur throughout the rRNA sequence of the chloro-ribosome (except for the conserved peptidyl transferase center region) but the overall length of the rRNAs do not change significantly, compared to the E. coli. Although, recent advancements in cryo-electron microscopy (cryo-EM) have provided detailed high-resolution structures of ribosomes from many different sources, a high-resolution structure of the chloro-ribosome is still lacking. Here, we present a cryo-EM structure of the large subunit of the chloro-ribosome from spinach (Spinacia oleracea) at an average resolution of 3.5 Å. High-resolution map enabled us to localize and model chloro-ribosome proteins, chloroplast-specific protein extensions, two PSRPs (PSRP5 and 6) and three rRNA molecules present in the chloro-ribosome. Although comparable to E. coli, the polypeptide tunnel and the tunnel exit site show chloroplast-specific features.
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