ArticleGenes, brain, and behavior2017
A new brain dopamine-deficient Drosophila and its pharmacological and genetic rescue.
Article in Genes, brain, and behavior, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Mating imperatives drive plasticity of the daily temporal niche in fruit flies.Nature ecology & evolution · 2026Article
- Hippo signaling regulates cuticle pigmentation and dopamine metabolism in Drosophila.PLoS genetics · 2026Article
- Non-uniform chromosomal SNP density biases sites of meiotic crossovers in Drosophila melanogaster.G3 (Bethesda, Md.) · 2026Article
- RNAi-based screen for pigmentation iniScience · 2026Article
- Reserpine prolongs lifespan but compromises locomotion and heat-stress resilience in Drosophila melanogaster.npj aging · 2026Article
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- Non-uniform chromosomal SNP density biases sites of meiotic crossovers inbioRxiv : the preprint server for biology · 2025Article
- Hippo signaling regulates cuticle pigmentation and dopamine metabolism inbioRxiv : the preprint server for biology · 2025Article
- RNAi-based screen for pigmentation inbioRxiv : the preprint server for biology · 2025Article
- Catecholamines Differ in Their Capacity to Form Melanin.microPublication biology · 2025Article
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- Flexible specificity of memory ineLife · 2023Article
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- Transcriptional responses are oriented towards different components of the rearing environment in two Drosophila sibling species.BMC genomics · 2022Article
- The Use ofBiomedicines · 2022Review
- The Role of the Dopamine Transporter in the Effects of Amphetamine on Sleep and Sleep Architecture in Drosophila.Neurochemical research · 2022Article
- A pair of dopamine neurons mediate chronic stress signals to induce learning deficit inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Drosophila tubulin polymerization promoting protein mutants reveal pathological correlates relevant to human Parkinson's disease.Scientific reports · 2021Article
- Associative learning in larval and adult Drosophila is impaired by the dopamine-synthesis inhibitor 3-Iodo-L-tyrosine.Biology open · 2021Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Dopamine (DA) is a neurotransmitter with conserved behavioral roles between invertebrate and vertebrate animals. In addition to its neural functions, in insects DA is a critical substrate for cuticle pigmentation and hardening. Drosophila tyrosine hydroxylase (DTH) is the rate limiting enzyme for DA biosynthesis. Viable brain DA-deficient flies were previously generated using tissue-selective GAL4-UAS binary expression rescue of a DTH null mutation and these flies show specific behavioral impairments. To circumvent the limitations of rescue via binary expression, here we achieve rescue utilizing genomically integrated mutant DTH. As expected, our DA-deficient flies have no detectable DTH or DA in the brain, and show reduced locomotor activity. This deficit can be rescued by l-DOPA/carbidopa feeding, similar to human Parkinson's disease treatment. Genetic rescue via GAL4/UAS-DTH was also successful, although this required the generation of a new UAS-DTH1 transgene devoid of most untranslated regions, as existing UAS-DTH transgenes express in the brain without a Gal4 driver via endogenous regulatory elements. A surprising finding of our newly constructed UAS-DTH1m is that it expresses DTH at an undetectable level when regulated by dopaminergic GAL4 drivers even when fully rescuing DA, indicating that DTH immunostaining is not necessarily a valid marker for DA expression. This finding necessitated optimizing DA immunohistochemistry, showing details of DA innervation to the mushroom body and the central complex. When DA rescue is limited to specific DA neurons, DA does not diffuse beyond the DTH-expressing terminals, such that DA signaling can be limited to very specific brain regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.