Evidence map›Paper›PMID 27761977›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2017

Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and schwann cell-like cells in a slow-flow microfluidic device.

Poornapriya Ramamurthy, Joshua B White, Joong Yull Park, Richard I Hume, Fumi Ebisu, Flor Mendez, Shuichi Takayama, Kate F Barald

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Advances in Microfluidic Cochlea-On-A-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Poornapriya RamamurthyDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan.
Joshua B WhiteDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan.
Joong Yull ParkSchool of Mechanical Engineering, College of Engineering, Chung-Ang University, Seoul, Republic of Korea.
Richard I HumeDepartment of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan.
Fumi EbisuDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.
Flor MendezDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan.
Shuichi TakayamaDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan.
Kate F BaraldDepartment of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan.
University of Michigan–Ann Arbor · USChung-Ang University · KR

Funding

Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
University of Michigan Postbaccalaureate Research Education Program (UM PREP)R25GM086262 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALLEN, BENJAMIN · 2009 to 2024
$6.1M
Microfluidics in Biomedical Sciences Training ProgramT32EB005582 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FAN, XUDONG · 2005 to 2020
$3.9M
MOLECULAR CONTROL OF PATTERNING IN EARLY EAR DEVELOPMENTR01DC004184 · NIDCD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BARALD, KATE FRANCESCA · 2000 to 2009
$2.7M
Knock-In Model for Inner Ear Hair Cell RegenerationR01DC005939 · NIDCD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BARALD, KATE FRANCESCA · 2002 to 2006
$1.7M
Signals Regulating SAG DevelopmentR01DC006436 · NIDCD · INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS · PI MARRS, JAMES A · 2004 to 2010
$1.6M
NIBIB NIH HHS T32 EB005582NIDCD NIH HHS R01 DC004184NIDCD NIH HHS R01 DC005939NIDCD NIH HHS R01 DC006436NIDCR NIH HHS T32 DE007057NIGMS NIH HHS R25 GM086262
6 · The paper itself

Abstract

backgroundTo send meaningful information to the brain, an inner ear cochlear implant (CI) must become closely coupled to as large and healthy a population of remaining spiral ganglion neurons (SGN) as possible. Inner ear gangliogenesis depends on macrophage migration inhibitory factor (MIF), a directionally attractant neurotrophic cytokine made by both Schwann and supporting cells (Bank et al., 2012). MIF-induced mouse embryonic stem cell (mESC)-derived "neurons" could potentially substitute for lost or damaged SGN. mESC-derived "Schwann cells" produce MIF, as do all Schwann cells (Huang et al., a; Roth et al., 2007; Roth et al., 2008) and could attract SGN to a "cell-coated" implant.

resultsNeuron- and Schwann cell-like cells were produced from a common population of mESCs in an ultra-slow-flow microfluidic device. As the populations interacted, "neurons" grew over the "Schwann cell" lawn, and early events in myelination were documented. Blocking MIF on the Schwann cell side greatly reduced directional neurite outgrowth. MIF-expressing "Schwann cells" were used to coat a CI: Mouse SGN and MIF-induced "neurons" grew directionally to the CI and to a wild-type but not MIF-knockout organ of Corti explant.

conclusionsTwo novel stem cell-based approaches for treating the problem of sensorineural hearing loss are described. Developmental Dynamics 246:7-27, 2017. © 2016 Wiley Periodicals, Inc.

Indexed as

Cell DifferentiationAnimalsCochlear ImplantsHearing LossIntramolecular OxidoreductasesLab-On-A-Chip DevicesMacrophage Migration-Inhibitory FactorsMiceMouse Embryonic Stem CellsMyelin SheathNeuronsSchwann CellsSpiral GanglionIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMif protein, mousecochlear implantdeafnessinner ear neuronsmicrofluidicsmyelinationSchwann cellsstem cells

Identifiers

PMID27761977
PMCPMC5159187
OpenAlexW2539343757

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.