ArticleScience China. Life sciences2016
Differential expression analysis of the broiler tracheal proteins responsible for the immune response and muscle contraction induced by high concentration of ammonia using iTRAQ-coupled 2D LC-MS/MS.
Article in Science China. Life sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Integrated Proteomics and Metabolomics Profiling Unravels Molecular Mechanisms Underlying Postmortem Meat Quality Between Two Ages and Muscle Types in Sansui Duck.Animals : an open access journal from MDPI · 2025Article
- Aerial ammonia exposure induces the perturbation of the interorgan ammonia disposal and branched-chain amino acid catabolism in growing pigs.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Protective effect of dietary supplementation of Bupleurum falcatum L saikosaponins on ammonia exposure-induced ileum injury in broilers.Poultry science · 2021Article
- Broilers' head behavior as an early warning index of production and lung health under ammonia exposure.Poultry science · 2021Article
- The alterations of tracheal microbiota and inflammation caused by different levels of ammonia exposure in broiler chickens.Poultry science · 2021Article
- Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits.PloS one · 2021Article
- Exposure to High Aerial Ammonia Causes Hindgut Dysbiotic Microbiota and Alterations of Microbiota-Derived Metabolites in Growing Pigs.Frontiers in nutrition · 2021Article
- Ammonia induce lung tissue injury in broilers by activating NLRP3 inflammasome via Escherichia/Shigella.Poultry science · 2020Article
- Effects of ammonia exposure on growth performance and cytokines in the serum, trachea, and ileum of broilers.Poultry science · 2020Article
- Ammonia Exposure Induced Cilia Dysfunction of Nasal Mucosa in the Piglets.BioMed research international · 2020Article
- Atmospheric Ammonia Affects Myofiber Development and Lipid Metabolism in Growing Pig Muscle.Animals : an open access journal from MDPI · 2019Article
- The Variation of Nasal Microbiota Caused by Low Levels of Gaseous Ammonia Exposure in Growing Pigs.Frontiers in microbiology · 2019Article
- Proteomic Analysis of Chicken Skeletal Muscle during Embryonic Development.Frontiers in physiology · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ammonia has been considered the contaminant primarily responsible for respiratory disease in poultry. Even though it can cause tracheal lesions, its adverse effects on the trachea have not been sufficiently studied. The present study investigated tracheal changes in Arbor Acres broilers (Gallus gallus) induced by high concentration of ammonia using isobaric tag for relative and absolute quantification (iTRAQ)-based proteome analysis. In total, 3,706 proteins within false discovery rate of 1% were identified, including 119 significantly differentially expressed proteins. Functional analysis revealed that proteins related to immune response and muscle contraction were significantly enriched. With respect to the immune response, up-regulated proteins (like FGA) were pro-inflammatory, while down-regulated proteins participated in antigen processing and antigen presenting (like MYO1G), immunoglobulin and cathelicidin production (like fowlicidin-2), and immunodeficiency (like PTPRC). Regarding muscle contraction, all differentially expressed proteins (like TPM1) were up-regulated. An over-expression of mucin, which is a common feature of airway disease, was also observed. Additionally, the transcriptional alterations of 6 selected proteins were analyzed by quantitative RT-PCR. Overall, proteomic changes suggested the onset of airway obstruction and diminished host defense in trachea after ammonia exposure. These results may serve as a valuable reference for future interventions against ammonia toxicity.
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