ArticleAnalytical and bioanalytical chemistry2017
Monitoring cell secretions on microfluidic chips using solid-phase extraction with mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Automated and online LC-MS/MS for multiplexed monitoring of hormone secretion from murine islets.Analytical and bioanalytical chemistry · 2026Article
- Online LC-MS/MS Analysis for Profiling Peptide Hormone Secretion Dynamics from Islets of Langerhans.Analytical chemistry · 2025Article
- Simultaneous monitoring of multiple hormones from human islets of Langerhans using solid-phase extraction-mass spectrometry.Analytical and bioanalytical chemistry · 2023Article
- Technological advances for analyzing the content of organ-on-a-chip by mass spectrometry.Frontiers in bioengineering and biotechnology · 2023Review
- Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes.Lab on a chip · 2022Article
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Rapid liquid chromatography-mass spectrometry quantitation of glucose-regulating hormones from human islets of Langerhans.Journal of chromatography. A · 2021Article
- A 3D human adipose tissue model within a microfluidic device.Lab on a chip · 2021Article
- Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution.Lab on a chip · 2020Article
- Dynamic in vitro intestinal barrier model coupled to chip-based liquid chromatography mass spectrometry for oral bioavailability studies.Analytical and bioanalytical chemistry · 2020Article
- Protein and Proteome Measurements with Microfluidic Chips.Analytical chemistry · 2020Review
- New Frontiers for Biofabrication and Bioreactor Design in Microphysiological System Development.Trends in biotechnology · 2019Review
- Review of 3D Cell Culture with Analysis in Microfluidic Systems.Analytical methods : advancing methods and applications · 2019Article
- 3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate.Lab on a chip · 2019Article
- Microfluidic Chip with Integrated Electrophoretic Immunoassay for Investigating Cell-Cell Interactions.Analytical chemistry · 2018Article
- Microfluidic systems for studying dynamic function of adipocytes and adipose tissue.Analytical and bioanalytical chemistry · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Microfluidics is an enabling technology for both cell biology and chemical analysis. We combine these attributes with a microfluidic device for on-line solid-phase extraction (SPE) and mass spectrometry (MS) analysis of secreted metabolites from living cells in culture on the chip. The device was constructed with polydimethylsiloxane (PDMS) and contains a reversibly sealed chamber for perfusing cells. A multilayer design allowed a series of valves to control an on-chip 7.5 μL injection loop downstream of the cell chamber with operation similar to a six-port valve. The valve collects sample and then diverts it to a packed SPE bed that was connected in-line to treat samples prior to MS analysis. The valve allows samples to be collected and injected onto the SPE bed while preventing exposure of cells to added back pressure from the SPE bed and organic solvents needed to elute collected chemicals. Here, cultured murine 3T3-L1 adipocytes were loaded into the cell chamber and non-esterified fatty acids (NEFAs) that were secreted by the cells were monitored by SPE-MS at 30 min intervals. The limit of detection for a palmitoleic acid standard was 1.4 μM. Due to the multiplexed detection capabilities of MS, a variety of NEFAs were detected. Upon stimulation with isoproterenol and forskolin, secretion of select NEFAs was elevated an average of 1.5-fold compared to basal levels. Despite the 30-min delay between sample injections, this device is a step towards a miniaturized system that allows automated monitoring and identification of a variety of molecules in the extracellular environment.
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