Evidence map›Paper›PMID 27761067›Full record

ArticlePharmacognosy magazine2016

Genetic and Chemical Profiling of

Ashutosh Kumar Verma, Sunita Singh Dhawan, Seema Singh, Kumar Avinash Bharati, Jyotsana

Abstract read
In one paragraph

Article in Pharmacognosy magazine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Chromatography Based Metabolomics andEvidence-based complementary and alternative medicine : eCAM · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ashutosh Kumar VermaCentral Botanical Laboratory, Botanical Survey of India, AJC Bose Indian Botanic Garden, Howrah, West Bengal, India.
Sunita Singh DhawanPlant Biotechnology Division, CSIR - Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Seema SinghPlant Genetics Unit, Department of Botany, University of Lucknow, Lucknow, Uttar Pradesh, India.
Kumar Avinash BharatiCentral National Herbarium, Botanical Survey of India, AJC Bose Indian Botanic Garden, Howrah, West Bengal, India.
JyotsanaAnalytical Chemistry Division, CSIR - Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background

objectiveStudy of genetic and chemical diversity and its implications in accessions of MATERIALS AND

methodsFourteen accessions of

resultsAmong the screened 40 ISSR primers, 15 were found polymorphic and collectively produced nine unique accession-specific bands. The maximum and minimum numbers of amplicones were noted for ISSR-15 and ISSR-11, respectively. The ISSR -11 and ISSR-13 revealed 100% polymorphism. HPLC chromatograms showed that accessions possess the secondary metabolites of mid-polarity with considerable variability. Unknown peaks with retention time 2.63, 3.41, 23.83, 24.50, and 44.67 were found universal type. Comparative hierarchical clustering analysis based on foresaid fingerprints indicates that both techniques have equal potential to discriminate accessions according to percentage gymnemic acid in their leaf tissue. Second approach was noted more efficiently for separation of accessions according to their agro-climatic/collection site.

conclusionHighly polymorphic ISSRs could be utilized as molecular probes for further selection of high gymnemic acid yielding accessions. Observed accession specific bands may be used as a descriptor for plant accessions protection and converted into sequence tagged sites markers. Identified five universal type peaks could be helpful in identification of SUMMARY: Nine accession specific unique bandsFive marker peaks for

Indexed as

Chemical fingerprintinggenetic fingerprintinggymnemic acidHPLCISSRmarker peaks

Identifiers

PMID27761067
PMCPMC5068116

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.