ArticleBiophysical journal2016
Arbitrary-Region Raster Image Correlation Spectroscopy.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Cytoskeletal control in adult microglia is essential to restore neurodevelopmental synaptic and cognitive deficits.Science advances · 2025Article
- Spectral phasor imaging on a commercial confocal microscope without a spectral detector.Scientific reports · 2025Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Hydration- and Temperature-Dependent Fluorescence Spectra of Laurdan Conformers in a DPPC Membrane.Cells · 2024Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- A change in biophysical properties accompanies heterochromatin formation in mouse embryos.Genes & development · 2023Article
- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Alterations induced by the PML-RARα oncogene revealed by image cross correlation spectroscopy.Biophysical journal · 2022Article
- Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration.BBA advances · 2022Article
- Mandipropamid as a chemical inducer of proximity for in vivo applications.Nature chemical biology · 2022Article
- Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection.eLife · 2021Article
- Article
- Measuring Nanoscale Distances by Structured Illumination Microscopy and Image Cross-Correlation Spectroscopy (SIM-ICCS).Sensors (Basel, Switzerland) · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Recent evolution of a TET-controlled and DPPA3/STELLA-driven pathway of passive DNA demethylation in mammals.Nature communications · 2020Article
- G1/S transcription factors assemble in increasing numbers of discrete clusters through G1 phase.The Journal of cell biology · 2020Article
- Fluorescence microscopy data for quantitative mobility and interaction analysis of proteins in living cells.Data in brief · 2020Article
- Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells.Molecular biology of the cell · 2020Article
- Live Cell Imaging Demonstrates Multiple Routes Toward a STAT1 Gain-of-Function Phenotype.Frontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Combining imaging with correlation spectroscopy, as in raster image correlation spectroscopy (RICS), makes it possible to extract molecular translational diffusion constants and absolute concentrations, and determine intermolecular interactions from single-channel or multicolor confocal laser-scanning microscopy (CLSM) images. Region-specific RICS analysis remains very challenging because correlations are always calculated in a square region-of-interest (ROI). In this study, we describe a generalized image correlation spectroscopy algorithm that accepts arbitrarily shaped ROIs. We show that an image series can be cleaned up before arbitrary-region RICS (ARICS) analysis. We demonstrate the power of ARICS by simultaneously measuring molecular mobility in the cell membrane and the cytosol. Mobility near dynamic subcellular structures can be investigated with ARICS by generating a dynamic ROI. Finally, we derive diffusion and concentration pseudo-maps using the ARICS method. ARICS is a powerful expansion of image correlation spectroscopy with the potential of becoming the new standard for extracting biophysical parameters from confocal fluorescence images.
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