ArticleBiophysical journal2016
Cellular Stiffness as a Novel Stemness Marker in the Corneal Limbus.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- High-throughput Genome Wide CRISPR Knock Out mechanical sort identifies genes driving metastatic cancer cell softening.bioRxiv : the preprint server for biology · 2026Article
- Gigantol Preserves Lens Biophysical Homeostasis by Restoring Cytoskeletal Integrity and Membrane Fluidity in a Diabetic Cataract Model.International journal of molecular sciences · 2026Article
- Real-time viscoelastic deformability cytometry: High-throughput mechanical phenotyping of liquid and solid biopsies.Science advances · 2024Article
- Recent advances of natural and bioengineered extracellular vesicles and their application in vascular regeneration.Regenerative biomaterials · 2022Review
- Regulation of Limbal Epithelial Stem Cells: Importance of the Niche.International journal of molecular sciences · 2021Review
- Biophysical properties of corneal cells reflect high myopia progression.Biophysical journal · 2021Article
- Biomechanical Modulation Therapy-A Stem Cell Therapy Without Stem Cells for the Treatment of Severe Ocular Burns.Translational vision science & technology · 2020Article
- Alterations in corneal biomechanics underlie early stages of autoimmune-mediated dry eye disease.Journal of autoimmunity · 2020Article
- Label-free microfluidic enrichment of photoreceptor cells.Experimental eye research · 2020Article
- Substrate stiffness induced mechanotransduction regulates temporal evolution of human fetal neural progenitor cell phenotype, differentiation, and biomechanics.Biomaterials science · 2020Article
- Stiffness based enrichment of leukemia cells using microfluidics.APL bioengineering · 2020Article
- M2 macrophage-derived exosomes promote the c-KIT phenotype of vascular smooth muscle cells during vascular tissue repair after intravascular stent implantation.Theranostics · 2020Article
- Brillouin microscopy: an emerging tool for mechanobiology.Nature methods · 2019Review
- Article
- Assessment of corneal substrate biomechanics and its effect on epithelial stem cell maintenance and differentiation.Nature communications · 2019Article
- Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules.Materials today (Kidlington, England) · 2018Article
- Microfluidic cell sorting by stiffness to examine heterogenic responses of cancer cells to chemotherapy.Cell death & disease · 2018Article
- Characterizing cellular mechanical phenotypes with mechano-node-pore sensing.Microsystems & nanoengineering · 2018Article
- Biophysical subsets of embryonic stem cells display distinct phenotypic and morphological signatures.PloS one · 2018Article
- Microfluidic Sorting of Cells by Viability Based on Differences in Cell Stiffness.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Healthy eyes contain a population of limbal stem cells (LSCs) that continuously renew the corneal epithelium. However, each year, 1 million Americans are afflicted with severely reduced visual acuity caused by corneal damage or disease, including LSC deficiency (LSCD). Recent advances in corneal transplant technology promise to repair the cornea by implanting healthy LSCs to encourage regeneration; however, success is limited to transplanted tissues that contain a sufficiently high percentage of LSCs. Attempts to screen limbal tissues for suitable implants using molecular stemness markers are confounded by the poorly understood signature of the LSC phenotype. For cells derived from the corneal limbus, we show that the performance of cell stiffness as a stemness indicator is on par with the performance of ΔNP63α, a common molecular marker. In combination with recent methods for sorting cells on a biophysical basis, the biomechanical stemness markers presented here may enable the rapid purification of LSCs from a heterogeneous population of corneal cells, thus potentially enabling clinicians and researchers to generate corneal transplants with sufficiently high fractions of LSCs, regardless of the LSC percentage in the donor tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.