ArticlePloS one2016
Pleomorphic Structures in Human Blood Are Red Blood Cell-Derived Microparticles, Not Bacteria.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- The Intratumoral Bacterial Metataxonomic Signature of Hepatocellular Carcinoma.Microbiology spectrum · 2022Article
- Human circulating bacteria and dysbiosis in non-infectious diseases.Frontiers in cellular and infection microbiology · 2022Review
- Morphology of blood microbiota in healthy individuals assessed by light and electron microscopy.Frontiers in cellular and infection microbiology · 2022Article
- Culturable and Non-Culturable Blood Microbiota of Healthy Individuals.Microorganisms · 2021Article
- Is There a Brain Microbiome?Neuroscience insights · 2021Review
- The Healthy Human Blood Microbiome: Fact or Fiction?Frontiers in cellular and infection microbiology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundRed blood cell (RBC) transfusions are a common, life-saving therapy for many patients, but they have also been associated with poor clinical outcomes. We identified unusual, pleomorphic structures in human RBC transfusion units by negative-stain electron microscopy that appeared identical to those previously reported to be bacteria in healthy human blood samples. The presence of viable, replicating bacteria in stored blood could explain poor outcomes in transfusion recipients and have major implications for transfusion medicine. Here, we investigated the possibility that these structures were bacteria.
resultsFlow cytometry, miRNA analysis, protein analysis, and additional electron microscopy studies strongly indicated that the pleomorphic structures in the supernatant of stored RBCs were RBC-derived microparticles (RMPs). Bacterial 16S rDNA PCR amplified from these samples were sequenced and was found to be highly similar to species that are known to commonly contaminate laboratory reagents.
conclusionsThese studies suggest that pleomorphic structures identified in human blood are RMPs and not bacteria, and they provide an example in which laboratory contaminants may can mislead investigators.
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Registered trials
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